Stable transformation of Erysiphe graminis, an obligate biotrophic pathogen of barley
Stable transformation of Erysiphe graminis, an obligate biotrophic pathogen of barley
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DOI：
10.1038/72666
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发表时间：
2000-02-01
影响因子：
46.9
通讯作者：
Spanu, P
中科院分区：
文献类型：
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作者：
Chaure, P;Gurr, SJ;Spanu, P
大麦白粉病Erysihe graminis f.sp.霍尔迪是一种专性的生物营养病原体，因此如果没有活的宿主，就不能完成其生活史。无法转化这种真菌并操纵其基因组，限制了对其生活史和致病性的研究。在这里，我们描述了一种基于使用金粒子枪传递DNA和使用苯菌灵或生物磷选择的植物内转化系统。用这种方法，我们一致地获得了稳定的转化子，其效率与其他丝状真菌相当。通过选择标记cc转化uidA基因，证明了β-葡萄糖醛酸苷酶在小麦赤霉菌中的稳定表达。
Barley powdery mildew, Erysiphe graminis f.sp. hordei, is an obligate biotrophic pathogen and as such cannot complete its life cycle without a living host. The inability to transform this fungus and manipulate its genome has constrained research towards understanding its life cycle and pathogenicity. Here we describe an in planta transformation system based on delivery of DNA using a gold-particle gun and selection using benomyl or bialaphos. Using this method, we consistently obtained stable transformants with efficiencies comparable to other filamentous fungi. Stable expression of the beta-glucuronidase in E. graminis was demonstrated by cc-transforming the uidA gene with the selectable markers.