Transforming growth factor-beta 1 differentially regulates proliferation, morphology, and extracellular matrix expression by three neural crest-derived neuroblastoma cell lines.
Transforming growth factor-beta 1 differentially regulates proliferation, morphology, and extracellular matrix expression by three neural crest-derived neuroblastoma cell lines.
复制标题
转化生长因子-β 1 差异性调节三种神经嵴源性神经母细胞瘤细胞系的增殖、形态和细胞外基质表达。
DOI：
10.1006/excr.1994.1085
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发表时间：
1994
影响因子：
3.7
通讯作者：
Krisinski,S
中科院分区：
文献类型：
--
作者：
Rogers,SL;Cutts,JL;Gegick,PJ;McGuire,PG;Rosenberger,C;Krisinski,S
我们以前报道过(S.L.Rogers，P.J.Gegick，S.M.Alexander和P.G.McGuire，Dev.Biol.151,191-203,1992)，转化生长因子-β1(转化生长因子-β1)在体外抑制了一个群体的鹌鹑神经脊细胞的增殖，上调了纤维连接蛋白的合成，并抑制了黑素的生成。在此，我们报道了来自亲本SK-N-SH神经母细胞瘤系(R.A.Ross，B.A.Spengler和J.L.Biedler，J.Natl.)的细胞系。癌症研究所)对转化生长因子β1的反应不同，他们的反应为进一步了解这种生长因子对神经脊亚群的作用提供了进一步的见解。SH-EP细胞株主要表现为非神经性特征，并对转化生长因子β-1有反应，培养6天后胸腺嘧啶核苷摄取增加，纤维连接蛋白基因和蛋白表达增加，层粘连蛋白合成减少。许多SH-EP细胞也获得了显著延长的形态，这让人想起培养中的雪旺细胞。神经性SY5Y细胞对胸腺嘧啶核苷的摄取没有明显改变。无论经转化生长因子β1处理或未处理的培养细胞均未检测到纤维连接蛋白基因和蛋白的表达，尽管层粘连蛋白的合成被生长因子上调。在转化生长因子β-1处理的SH-IN中间细胞系的培养中，许多细胞明显扁平，胸腺嘧啶核苷摄取量稳定下降，纤维连接蛋白和层粘连蛋白的表达增加。观察到的SH-IN细胞的反应类似于在原代神经脊培养中观察到的反应，并且似乎表现出类似的向间充质表型分化。这些结果证实了这样一种观点，即密切相关但不同的神经脊来源的细胞类型对转化生长因子β1有选择性的反应，并表明这些SK-N-SH来源的细胞系将在实验方法中有用，使我们能够推断神经脊分化的潜在调控机制。
We reported previously (S. L. Rogers, P. J. Gegick, S. M. Alexander, and P. G. McGuire,Dev. Biol.151, 191-203, 1992) that transforming growth factor-β1 (TGFβ1) inhibited proliferation, up-regulated fibronectin synthesis, and suppressed melanogenesis in a population of quail neural crest cellsin vitro. Here, we report that cell lines derived from the parent SK-N-SH neuroblastoma line (R. A. Ross, B. A. Spengler, and J. L. Biedler,J. Natl. Cancer Inst.71, 741-747, 1983) respond differentially to TGFβ1, and their responses provide further insights into the actions of this growth factor on neural crest subpopulations. The SH-EP cell line exhibits primarily nonneuronal traits and responded to TGFβ1 with increased thymidine uptake after 6 days of culture, increased expression of fibronectin mRNA and protein, and decreased laminin synthesis. Many SH-EP cells also acquired a dramatically elongated morphology, reminiscent of Schwann cells in culture. Thymidine uptake by the neuronal SY5Y cell line was not substantially altered. Neither fibronectin mRNA nor protein was detectable in either TGFβ1-treated or untreated cultures, although laminin synthesis was upregulated by the growth factor. In TGFβ1-treated cultures of the intermediate SH-IN cell line, which has been reported to display both neuronal and nonneuronal characteristics, there was marked flattening of many cells, a steady decrease in thymidine uptake, and increased expression of both fibronectin and laminin. The observed responses of SH-IN cells mimic those observed in primary neural crest cultures and appear to represent similar differentiation toward a mesenchymal phenotype. These results substantiate the idea that closely related but diverging neural crest-derived cell types respond selectively to TGFβ1 and demonstrate that these SK-N-SH-derived cell lines will be useful in experimental approaches that will allow us to infer mechanisms underlying regulation of neural crest differentiation.