Applications of a mouse model of calvarial healing: Differences in regenerative abilities of juveniles and adults
Applications of a mouse model of calvarial healing: Differences in regenerative abilities of juveniles and adults
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DOI：
10.1097/01.prs.0000131016.12754.30
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发表时间：
2004-09-01
影响因子：
3.6
通讯作者：
Longaker, MT
中科院分区：
文献类型：
--
作者：
Aalami, OO;Nacamuli, RP;Longaker, MT
幼儿能够修复巨大的颅骨缺陷，而成年人缺乏这种内源性的骨组织工程能力。尽管有重要的临床意义，但人们对这种差异能力背后的分子和细胞生物学知之甚少。传统上，豚鼠、兔和大鼠模型被用来研究颅骨愈合的协调。为了利用基因敲除和转基因小鼠的研究潜力，作者开发了一个颅骨愈合的小鼠模型。分别在幼龄(6天龄，n=15)和成年(60天龄，n=15)小鼠身上制作直径分别为3、4和5 mm的非缝合相关顶骨缺损。8周后取颅盖骨，进行放射学和组织学分析。使用Scion Linage软件对颅骨X线片进行分析，量化愈合率。当产生3 mm、4 mm或5 mm的缺陷时，6天大的小鼠和60天大的小鼠在修复颅骨缺陷的能力方面存在显著差异。作者的分析表明，幼年小鼠的颅骨缺陷愈合比例明显高于成年小鼠(幼年平均愈合百分比：3 mm缺陷，59%；4 mm缺陷，65%；5 mm缺陷，44%；成年平均愈合百分比：
Young children are capable of healing large calvarial defects, whereas adults lack this endogenous osseous tissue-engineering capacity. Despite the important clinical implications, little is known about the molecular and cell biology underlying this differential ability. Traditionally, guinea pig, rabbit, and rat models have been used to study the orchestration of calvarial healing. To harness the research potential of knockout and transgenic mice, the authors developed a mouse model for calvarial healing. Nonsuture-associated parietal defects 3, 4, and 5 mm in diameter were made in both juvenile (6-day-old, n = 15) and adult (60-day-old, n = 15) mice. Calvariae were harvested after 8 weeks and analyzed radiographically and histologically. Percentage of healing was quantified using Scion linage software analysis of calvarial radiographs. A significant difference in the ability to heal calvarial defects was seen between 6-day-old and 60-day-old mice when 3-, 4-, or 5-mm defects were created. The authors' analysis revealed that juvenile mice healed a significantly greater percentage of their calvarial defects than adult mice (juvenile mean percentage of healing: 3-mm defects, 59 percent; 4-mm defects, 65 percent; 5-mm defects, 44 percent; adult mean percentage of healing: