Cloning and identification of porcine programmed death 1
Cloning and identification of porcine programmed death 1
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DOI：
10.1016/j.vetimm.2010.03.001
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发表时间：
2010-07-15
影响因子：
1.8
通讯作者：
Tong, Guang-Zhi
中科院分区：
文献类型：
--
作者：
Peng, Jin-Mei;Tian, Zhi-Jun;Tong, Guang-Zhi
程序性死亡1（PD-1）是免疫球蛋白（IG）超家族的成员，其在活化的T细胞、B细胞和单核细胞上表达。许多研究表明PD-1的高表达与某些人类感染的持续感染和免疫逃避密切相关。为了研究PD-1基因的表达与猪病毒持续感染的关系，我们首先根据EST数据库中的blast结果克隆了猪PD-1基因。序列分析表明，克隆的PD-1分子与人和鼠PD-1分别具有63%和54%的氨基酸序列同一性。其分子结构也与人和鼠PD-1相似，在胞外区含有IgV样结构域，在胞质尾区含有两个免疫调节基序。体外T细胞增殖实验表明，PD-1对猪T细胞增殖的抑制率为71%，对INF-γ和IL-2的分泌的抑制率分别为64%和53%。这些数据表明，猪PD-1以与其在人和小鼠免疫系统中的对应物相似的方式负调节猪免疫应答。(C)2010 Elsevier B. V.保留所有权利。
Programmed death 1 (PD-1) is a member of the immunoglobulin (Ig) superfamily, which is expressed on activated T cells, B cells and monocytes. Many researches have demonstrated that a high level of PD-1 expression is closely related to persistent infection and immune evasion in some human infections. In order to study the relationship between PD-1 expression and persistent infections caused by some porcine viruses, we first cloned the porcine PD-1 from porcine PBMCs based on the blast result in the EST database using the human PD-1 sequence. Sequence analysis showed that the cloned PD-1 molecule shares 63 and 54% amino acid sequence identity with human and murine PD-1, respectively. Its molecular structure is also similar to that of human and murine PD-1, containing an IgV-like domain in the extracellular region and two immune regulatory motifs in its cytoplasmic tail. The in vitro T cell proliferation assay showed that the cloned PD-1 could inhibit porcine T cell proliferation by 71% and secretion of INF-gamma and IL-2 by 64 and 53%, respectively. These data suggest that porcine PD-1 negatively regulates the porcine immune response in a similar manner to that of its counterpart in the human and mouse immune system. (C) 2010 Elsevier B.V. All rights reserved.