Multiplex analysis of cytokines as biomarkers that differentiate benign and malignant thyroid diseases.
Multiplex analysis of cytokines as biomarkers that differentiate benign and malignant thyroid diseases.
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DOI：
10.1002/prca.200780095
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发表时间：
2008-10-10
影响因子：
2
通讯作者：
Stack, Brendan C., Jr.
中科院分区：
文献类型：
--
作者：
Linkov, Faina;Ferris, Robert L.;Yurkovetsky, Zoya;Marrangoni, Adele;Velikokhatnaya, Lyudmila;Gooding, William;Nolan, Brian;Winans, Matthew;Siegel, Eric R.;Lokshin, Anna;Stack, Brendan C., Jr.
甲状腺癌的发病率正在增加，其诊断可能具有挑战性。细针活检是进行组织诊断的主要临床工具，在高达30%的病例中会导致不确定的诊断，从而导致手术。蛋白质组学的进展正在提高使用小样本组织或体液诊断恶性疾病的能力。我们假设，对血清生长因子的分析将揭示良性和恶性甲状腺疾病之间的诊断信息差异。使用xMAP图谱，我们评估了19种细胞因子、趋化因子和生长因子的浓度。我们用23例癌症患者(恶性组)、24例良性结节性甲状腺疾病患者(良性组)和23例健康人(正常组)的血清进行检测。在单变量分析中，5个因子(上皮生长因子、肝细胞生长因子、白细胞介素5和8、活化调节T细胞正常表达和推测分泌因子RANTES)将甲状腺疾病患者与正常组区分开来。在多因素分析中，SET{IL-8、肝细胞生长因子、单核细胞诱生的γ干扰素、IL-12 p40}在良、恶性组之间有显著差异(受试者工作特征曲线下面积为0.81(95%可信区间：0.65~0.90))。血清生物标记物的多重面板可能是诊断有结节性甲状腺疾病证据的患者的癌症的有前景的工具。
Thyroid cancer incidence is increasing, and its diagnosis can be challenging. Fine needle biopsy, the principal clinical tool to make a tissue diagnosis, leads to inconclusive diagnoses in up to 30% of the cases, leading to surgery. Advances in proteomics are improving abilities to diagnose malignant conditions using small samples of tissue or body fluids. We hypothesized that analysis of serum growth factors would uncover diagnostically informative differences between benign and malignant thyroid conditions. Using xMAP profiling, we evaluated concentrations of 19 cytokines, chemokines, and growth factors. We used sera from 23 patients with cancer (Malignant group), 24 patients with benign nodular thyroid disease (Benign group), and 23 healthy subjects (Normal group). In univariate analysis, five factors (epithelial growth factor, hepatocyte growth factor, Interleukins-5 and -8, and regulated upon activation, normally T-expressed and presumably secreted (RANTES) distinguished subjects with thyroid disease from the Normal group. In multivariate analysis, the set {Interleukin-8, hepatocyte growth factor, monocyte-induced γ interferon, interleukin-12 p40} achieved noteworthy discrimination between Benign and Malignant groups (area under the receiver operating characteristics curve was 0.81 (95% confidence interval: 0.65–0.90)). Multiplex panels of serum biomarkers may be promising tools to diagnose cancer in patients presenting with evidence of nodular thyroid disease.
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影响因子：
9.3
作者：
Bossuyt, PM;Reitsma, JB;de Vet, HCW
通讯作者：
de Vet, HCW
影响因子：
3.8
作者：
Linkov, Faina;Lisovich, Alex;Ferris, Robert L.
通讯作者：
Ferris, Robert L.
DOI：
10.1073/pnas.0503039102
发表时间：
2005-10-11
影响因子：
11.1
作者：
Borrello, MG;Alberti, L;Pierotti, MA
通讯作者：
Pierotti, MA
DOI：
10.1002/hed.20829
发表时间：
2008-08-01
影响因子：
2.9
作者：
Khalid, Ayesha N.;Quraishi, Sadeq A.;Stack, Brendan C., Jr.
通讯作者：
Stack, Brendan C., Jr.
影响因子：
11.5
作者：
Couch, Marion E.;Ferris, Robert L.;Sidransky, David
通讯作者：
Sidransky, David