Molecular Biology, Pathogenicity, and Ecology of Bacterial Plasmids
Molecular Biology, Pathogenicity, and Ecology of Bacterial Plasmids
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细菌质粒的分子生物学、致病性和生态学
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
E. Koenig
中科院分区：
文献类型：
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作者：
S. Levy;R. Clowes;E. Koenig
书名/作者The most important [M. J. Malamy 2 I医学系和2分子生物学和微生物学系，塔夫茨大学医学院，波士顿，MA 02111介绍了脆弱拟杆菌TMP 10的克林霉素-红霉素（clinr）耐药转移因子的特性。转移能力和clinr的决定子存在于一个名为pBFTM 10的15.6内切酶质粒上。最近在芝加哥分离出了脆弱拟杆菌的克林霉素和四环素耐药菌株。芝加哥tetr分离株TMP 230同时转移clinr和tetr，但用标准方法检测时似乎不含质粒。用Southern杂交技术证实了clinr转移因子pBF TM 10与TMP 230的染色体同源。clinr决定簇在染色体上的位置和转移方式正在调查中。厌氧细菌是人体正常植物群的重要成员;在结肠厌氧生物体中，包括拟杆菌属、梭菌属和无芽孢杆菌属，革兰氏阳性杆菌的数量超过兼性细菌，如！。大肠杆菌和粪链球菌的比例约为1000：1（1）。在过去的20-25年中，厌氧菌越来越被认为是人类化脓性感染的重要病原体（2）。脆弱拟杆菌是腹部、外科和妇科感染中最重要的厌氧菌，因为它在这种情况下最常侵入血流。大多数~。脆壁菌属菌株对中等水平的青霉素G和头孢菌素具有抗性;它们对氨基糖苷类抗生素具有一致的抗性，并且在20世纪60年代注意到出现了对四环素的广泛抗性（3，4）。后一种耐药性很重要，因为四环素是首选的药物
F.P. Tally] M.J. Shimell]G;R.Carson2and M.H. Malamy2 IDepartment of Medicine and 2Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111 The characteristics of the clindamycin-erythromycin (clinr) resistance transfer factor from Bacteroides fragilis TMP 10 are presented. Transfer ability and the determinant for clinr are found on a 15.6 kilobase plasmid named pBFTM 10. Recent clindamycin and tetracycline resistant strains of Bacteroides fragilis have been isolated in Chicago. The Chicago tetr isolate, TMP 230, transfers both clinr and tetr , but appearsto be plasmid free when tested by standard methods. Homology between the clinr transfer factor pBFTM 10 and the chromosome of the TMP 230 could be demonstrated by the Southern hybridization technique. The location of the clinr determinant on the chromosome and mode of transfer are under invistigation. Anaerobic bacteria are prominent members of the normal flora of man; in the colon anaerobic organisms including Bacteroides, Clostridia and non-sporing, gram-positive bacilli outnumber facultative bacteria such as!. coli and Streptococcus fecaelis by about 1000:1 (1). Over the past 20-25 years anaerobic bacteria have been increasingly recognized as important pathogens in human suppurative infections (2). Bacteroides fragilis emerges as the most important anaerobic bacterium in abdominal, surgical and gynecoiogical infections because it most frequently invades the bloodstream in this setting. Most~. fragilis strains are resistant to intermediate levels of penicillin G and cephalosporins; they are uniformly resistant to the amino glycoside antibiotics, and in the 1960's it was noted that there was the emergence of widespread resistance to tetracycline (3,4). This latter resistance was important because tetracycline was the agent of choice