Serum carboxypeptidase A activity as a biomarker for early-stage pancreatic carcinoma
Serum carboxypeptidase A activity as a biomarker for early-stage pancreatic carcinoma
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DOI：
10.1016/j.cca.2006.11.010
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发表时间：
2007-03-01
影响因子：
5
通讯作者：
Satomura, Shinji
中科院分区：
文献类型：
--
作者：
Matsugi, Shinji;Hamada, Takatoshi;Satomura, Shinji
背景：早期胰腺癌缺乏典型的临床症状和体征，诊断困难。我们建立了一种测定血清活性形式羧肽酶A（F-CPA）及其酶原前体前体CPA的方法，并评估它们作为早期胰腺癌的标志物。研究方法：本文以N-乙酰苯丙氨酰-L-3-硫代苯丙氨酸为底物，DL-苄基琥珀酸为CPA特异性抑制剂，测定了406例胰腺癌、53例急性胰腺炎、23例慢性胰腺炎和161例非胰腺疾病患者血清CPA。使用胰蛋白酶进行pro CPA的活化。结果：所建立的检测系统完全自动化，具有足够的性能，为临床常规检测。该系统需要31分钟用于CPA检测。胰腺癌患者与非胰腺疾病患者之间F-CPA活性无显著差异（p = 0. 05）。168）。F-CPA主要在胰腺炎患者中升高。胰腺癌和急性胰腺炎患者的总CPA（T-CPA，T-CPA = pm CPA + F-CPA）均显著升高（P <0. 05）。000 1，每例），仅胰腺癌患者的F-CPA/T-CPA比值较低。T-CPA在肿瘤<2cm的早期胰腺癌中的阳性率为77%，高于CA 19 - 9（31%）、CEA（8%）和弹性蛋白酶1（46%）。结论：血清T-CPA和F-CPA联合检测对胰腺癌的早期诊断有重要意义。(c)2006 Elsevier B. V保留所有权利。
Background: The early stage of pancreatic carcinoma lacks typical clinical signs and symptoms, and is difficult to diagnose. We developed an assay for active form of serum carboxypeptidase A (F-CPA) and its zymogen precursor pro CPA, and evaluated them as a marker for early-stage pancreatic carcinoma. Methods: Serum CPA from 406 patients including 169 with pancreatic carcinoma, 53 with acute pancreatitis, 23 with chronic pancreatitis, and 161 with non-pancreatic diseases were assayed by a method with N-acetyl-phenylalanyl-L-3-thiaphenylalanine as substrate and DL-benzylsuccinic acid as specific inhibitor of CPA. Activation of pro CPA was carried out using trypsin. Results: An established assay system was performed fully automatically and possesses enough performance for routine clinical assay. This system requires 31 minutes for CPA detection. No significant differences were detected between the F-CPA activity of patients with pancreatic carcinoma and that of patients with non-pancreatic diseases (p = 0. 168). F-CPA was mainly increased in patients with pancreatitis. Since total-CPA (T-CPA, T-CPA = pm CPA + F-CPA) was increased both in patients with pancreatic carcinoma and those with acute pancreatitis (p < 0. 000 1, each case), the F-CPA/T-CPA ratio was low only in the patients with pancreatic carcinoma. The rate of positivity of T-CPA in the early stage of pancreatic carcinoma in which tumor was less than 2 cm was 77%, and higher than those of CA19-9 (31%), CEA (8%), and elastase 1 (46%). Conclusion: It was suggested that assays of both T-CPA and F-CPA in serum might be useful for the surveillance of early-stage pancreatic carcinoma. (c) 2006 Elsevier B.V All rights reserved.