AUTORADIOGRAPHY USING STORAGE PHOSPHOR TECHNOLOGY

AUTORADIOGRAPHY USING STORAGE PHOSPHOR TECHNOLOGY
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DOI:
10.1002/elps.1150110503
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发表时间:
1990-05-01
期刊:
影响因子:
2.9
通讯作者:
BARKER, DL
BARKER, DL
中科院分区:
生物学3区
文献类型:
--
作者:
JOHNSTON, RF;PICKETT, SC;BARKER, DL

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我们描述了光激励存储磷光体成像板在用 32P、14C 和 35S 标记的样品放射自显影中的应用。成像板可以在 1 崩解/mm2 的曝光水平下检测 32P 标记的样品,在 25 崩解/mm2 的曝光水平下检测 14C 和 35S。与 X 射线胶片相比,成像板的 32P 灵敏度提高了 15 至 250 倍,14C 和 35S 灵敏度提高了 20 至 100 倍。分辨率在 0.3 毫米范围内,因此适用于任何凝胶或印迹应用。
We describe the application of photostimulable storage phosphor imaging plates to autoradiography of samples labeled with 32P, 14C and 35S. Imaging plates can detect a 32P-labeled sample at an exposure level of 1 disintegration/mm2, and 14C and 35S at 25 disintegrations/mm2. Compared to X-ray film, imaging plates offer increased sensitivity of 15- to 250-fold 32P and 20- to 100-fold for 14C and 35S. Resolution is in the 0.3 mm range and is therefore suitable for any gel or blot application.