SOURCE AND CONCENTRATION OF EXTRACELLULAR ADENOSINE-TRIPHOSPHATE DURING HEMOSTASIS IN RATS, RABBITS AND MAN
SOURCE AND CONCENTRATION OF EXTRACELLULAR ADENOSINE-TRIPHOSPHATE DURING HEMOSTASIS IN RATS, RABBITS AND MAN
复制标题
DOI：
10.1113/jphysiol.1984.sp015385
复制
发表时间：
1984-01-01
影响因子：
5.5
通讯作者：
KRATZER, MAA
中科院分区：
文献类型：
--
作者：
BORN, GVR;KRATZER, MAA
建立了一种新的测定微量全血细胞外游离ATP的方法-荧光素酶法。该方法具有非常低的背景，对应于. apprx。10-16 mol ATP。在大鼠和家兔动脉精确穿刺后止血期间以及Simplate器械对人体皮肤进行标准化切口后，测量血液中出现的ATP。血管损伤后2-4秒出现的血液中游离ATP的初始浓度约为血管损伤后2-4秒的2倍。10-7在大鼠和兔中为约2x M，10-6人类的M。游离ATP浓度增加到2 × 10 - 4。10-5损伤后3-5 min，M1/M2增加，肝素（20 U/ml）可抑制M1/M2的增加。最初游离ATP的来源被确定为受损血管壁中的受损细胞。足够的ADP，无论是与ATP一起释放还是由ATP的酶促去磷酸化产生，都将存在于损伤部位，以启动血小板的止血聚集。
A new technique was developed for the measurement of extracellular free ATP in very small samples of whole blood using the luciferin-luciferase enzyme system. The method had a very low background corresponding to .apprx. 10-16 mol ATP. ATP was measured in blood as it emerged during hemostasis following precise puncture of rat and rabbit arteries and after standardized incisions of human skin by the Simplate device. The initial concentration of free ATP in blood emerging 2-4 s after vascular injury was about 2 .times. 10-7 M in rats and rabbits and about 2 .times. 10-6 M in humans. The free-ATP concentration increased to 2 .times. 10-5 M 3-5 min after injury, and these increases could be prevented by heparin (20 U/ml). The source of the initial free ATP was identified as damaged cells in the injured vessel wall. Sufficient ADP, both released as such with ATP and generated by enzymic dephosphorylation of ATP, would be present at the site of injury to initiate hemostatic aggregation of platelets.