Evaluation of the NanoChip 400 system for detection of influenza A and B, respiratory syncytial, and parainfluenza viruses
Evaluation of the NanoChip 400 system for detection of influenza A and B, respiratory syncytial, and parainfluenza viruses
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DOI：
10.1128/jcm.01947-07
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发表时间：
2008-05-01
影响因子：
9.4
通讯作者：
Patterson, Bruce K.
中科院分区：
文献类型：
--
作者：
Takahashi, Hiroshi;Norman, Sylvia A.;Patterson, Bruce K.
NanoChip400系统使用多重PCR化学和电子芯片检测甲型和乙型流感病毒；呼吸道合胞病毒A和B；人类副流感病毒1 2 3型。将NanoChip 400系统获得的结果与直接荧光抗体染色（DFA）和实时PCR获得的结果分别对122和130个标本进行比较。122个样本中有106个（86.9%）的DFA与NanoChip 400系统的一致性。通过与可用于验证性实时PCR检测的标本的差异分析，NanoChip 400的灵敏度和特异性分别为98.6%和100%。与之前用实时PCR检测的标本相比，NanoChip 400系统的灵敏度为91.1%，特异性为100%。NanoChip 400系统为临床实验室提供了一种实用、快速、灵敏的检测常见呼吸道病毒的方法。
The NanoChip400 system uses multiplex PCR chemistry and electronic microarray detection of influenza A and B viruses; respiratory syncytial viruses A and B; and human parainfluenza virus types 1, 2, and 3. The results obtained with the NanoChip 400 system were compared with those obtained by direct fluorescent-antibody staining (DFA) and real-time PCR with 122 and 130 specimens, respectively. Concordance between DFA and NanoChip 400 system was obtained for 106 of 122 (86.9%) specimens. On the basis of discrepancy analysis with specimens available for confirmatory real-time PCR testing, the sensitivity and specificity of the NanoChip 400 were 98.6% and 100%, respectively. With respect to specimens previously tested by real-time PCR, the NanoChip 400 system demonstrated a sensitivity of 91.1% and a specificity of 100%. The NanoChip 400 system provides clinical laboratories with a practical, rapid, and sensitive method for the detection of common respiratory viruses.