Imaging axonal transport of mitochondria in vivo
Imaging axonal transport of mitochondria in vivo
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DOI：
10.1038/nmeth1055
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发表时间：
2007-07-01
期刊：
影响因子：
48
通讯作者：
Lichtman, Jeff W.
中科院分区：
文献类型：
--
作者：
Misgeld, Thomas;Kerschensteiner, Martin;Lichtman, Jeff W.
神经元线粒体调节突触生理和细胞生存，其功能或运输的中断会导致神经系统疾病。我们提出了一种荧光方法来选择性地成像小鼠神经系统中的线粒体动态，包括活的小鼠和急性外植体。我们发现轴突的损伤和恢复导致了顺行和逆行运输的早期和持续的改变。线粒体的活体成像将是分析这种重要细胞器的有用工具。
Neuronal mitochondria regulate synaptic physiology and cellular survival, and disruption of their function or transport causes neurological disease. We present a fluorescence method to selectively image mitochondrial dynamics in the mouse nervous system, in both live mice and acute explants. We show that axon damage and recovery lead to early and sustained changes in anterograde and retrograde transport. In vivo imaging of mitochondria will be a useful tool to analyze this essential organelle.