Use of cryopreserved rat arteries for microsurgical training
Use of cryopreserved rat arteries for microsurgical training
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DOI：
10.1002/micr.20153
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发表时间：
2005-01-01
期刊：
影响因子：
2.1
通讯作者：
Raimondi, JC
中科院分区：
文献类型：
--
作者：
Lausada, NR;Escudero, E;Raimondi, JC
硅橡胶管用作进行微血管吻合的训练材料。然而，硅橡胶的纹理不同于实际血管的纹理。在目前的工作中，我们评估使用保存的大鼠动脉段训练微血管闭塞。从股动脉、颈动脉和腹部动脉获得一厘米长的大鼠动脉段，保存在冷盐水溶液中并冷冻。受训者使用上述材料进行微血管扩张术，并回答关于质地、稠度和壁对针的阻力的问题，比较保存的动脉壁和硅橡胶管。他们还被问及动脉蒂是否具有与正常血管相似的稠度和纹理，以及它们是否是比合成材料更可靠的显微外科技术练习方法。他们更喜欢冷冻的动脉蒂而不是硅橡胶管。我们的结论是，动脉尸体段是一个合适的生物材料显微外科培训。由于它们可以从其他实验中获得，这是减少用于教学目的的繁殖和牺牲动物数量的有效方法。(c)2005 Wiley-Liss，Inc.
Silastic tubes are used as training material for performing microvascular anastomoses. However, silastic texture differs from that of actual blood vessels. In the present work, we evaluate the use of preserved rat arterial segments for training in microvascular anastomoses. One-centimeter-long rat arterial segments were obtained from femoral, carotid, and abdominal arteries, preserved in cold saline solution, and frozen. Trainees performed microvascular anastomoses using the aforementioned material and answered questions about texture, consistency, and wall resistence to the needle, comparing preserved arterial wall and silastic tubes. They were also asked whether the arterial pedicles had a consistency and texture similar to normal vessels, and if they were a more reliable method for practicing microsurgery techniques than synthetic materials. They preferred frozen arterial pedicles over silastic tubes. We conclude that arterial cadaveric segments are a suitable biologic material for microsurgical training. Since they can be obtained from other experiments, this is an effective way to reduce the number of animals bred and sacrificed for teaching purposes. (c) 2005 Wiley-Liss, Inc.