Alterations of neutrophil L-selectin and CD18 expression by tobacco smoke: implications for periodontal diseases.
Alterations of neutrophil L-selectin and CD18 expression by tobacco smoke: implications for periodontal diseases.
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烟草烟雾对中性粒细胞 L-选择素和 CD18 表达的改变：对牙周病的影响。
DOI：
10.1111/j.1600-0765.1998.tb02211.x
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发表时间：
1998
影响因子：
3.5
通讯作者：
Hyun,W
中科院分区：
文献类型：
--
作者：
Ryder,MI;Fujitaki,R;Lebus,S;Mahboub,M;Faia,B;Muhaimin,D;Hamada,M;Hyun,W
在吸烟过程中，慢性低水平烟草和急性短期高水平烟草烟雾引起的中性粒细胞功能改变，可能在吸烟者牙周疾病的发病机制中发挥作用。中性粒细胞功能的早期迁移事件之一是L-选择素和CD11/18整合素表面表达的改变。在目前的研究中，我们研究了玻璃烟雾暴露和尼古丁单独对吸烟者和非吸烟者中性粒细胞中这两种黏附分子表达的影响。我们还通过评估这一体外系统中尼古丁的暴露水平，并将这些水平与唾液和牙周沟液中的急性和慢性尼古丁水平进行比较，确定了这一体外系统的生理相关性。从吸烟者(1包/天)和不吸烟者的血液中分离外周中性粒细胞，分别与香烟烟雾(0-5分钟)、10−7M F-Met-Leu-Phe或单独尼古丁1.62 mg/ml至162 ng/ml(10−2M-10−6M)体外培养。然后将中性粒细胞与荧光标记的抗亮氨酸8抗体(L-选择素)、抗CD18整合素(CD18整合素)或γ-4(非特异性对照)共同孵育，固定后进行流式细胞仪分析。在香烟烟雾暴露下，吸烟者和非吸烟者在烟雾暴露1-5分钟时，L-选择素的脱落率约为75%，组间无显著差异。香烟烟雾暴露导致吸烟者和不吸烟者CD18表达增加15-20%。在所有时间点，与吸烟者相比，非吸烟者中CD18整合素的表达略高，但在统计学上并不显著。这些CD_(18)升高和L-选择素脱落的模式与10−7M F-Met-Leu-Phe孵育的大小相似。急性烟雾暴露后5min烟盒中尼古丁含量为529 ng/ml，唾液中尼古丁含量由吸烟前的109.2 ng/ml升至吸烟后的1821.4 ng/ml，龈沟液中的尼古丁含量在吸烟后升至5961 ng/ml。Vitronicotine在1.62~162 ng/ml范围内对L-选择素和CD18的表达无明显影响。
Alterations in neutrophil functions by both chronic low levels of tobacco and by acute short‐term higher levels of tobacco smoke, as encountered during the act of smoking, may play a role in the pathogenesis of periodontal diseases in smokers. Among the early migration events of neutrophil function is the alteration in surface expression of L‐selectin and the CD11/18 integrins. In the present study we examined the effect ofin vitrosmoke exposure and nicotine alone on the expression of these 2 adhesion molecules in neutrophils from smokers and non‐smokers. We also determined the physiological relevance of thisin vitrosystem by assessing the levels of nicotine exposure in thisin vitrosystem and comparing these levels to acute and chronic levels of nicotine in saliva and gingival crevicular fluid. Peripheral neutrophils were isolated from the blood of smokers (> 1 pack/d) and non‐smokers and incubatedin vitrowith either cigarette smoke (0–5 min), 10−7M F‐met‐leu‐phe, or nicotine alone at 1.62mg/ml to 162ng/ml (10−2M‐10−6M). The neutrophils were then incubated with fluoresceine conjugated anti‐Leu8 (L‐selectin), anti‐CD18 (CD18 integrin), or γ‐4 (non‐specific control), fixed and analyzed by flow cytometry. With cigarette smoke exposure, there was an approximate 75% shedding of L‐selectin in both smokers and non‐smokers with no marked difference between groups at 1–5 min of smoke exposure. Cigarette smoke exposure resulted in a 15–20% increase in CD 18 expression in both smokers and non‐smokers. At all time points, there was slightly greater but statistically insignificant expression of CD18 integrin in non‐smokers when compared to smokers. These patterns of CD18 increases and L‐selectin shedding were similar in magnitude to incubations with 10−7M F‐met‐leu‐phe. Acute smoke exposure resulted in elevation of nicotine in the smoke box to 529 ng/ml at 5 min, in saliva from 109.2 ng/ml before smoking to 1821.4 ng/ml after smoking, and in gingival crevicular fluid to 5961 ng/ml after smoking. No significant alterations in L‐selectin or CD 18 expression were noted within vitronicotine from 1.62 mg/ml to 162 ng/ml.