SOURCES OF EXPERIMENTAL VARIATION IN CALIBRATION CURVES FOR ENZYME-LINKED-IMMUNOSORBENT-ASSAY
SOURCES OF EXPERIMENTAL VARIATION IN CALIBRATION CURVES FOR ENZYME-LINKED-IMMUNOSORBENT-ASSAY
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DOI：
10.1016/0003-2670(95)00249-y
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发表时间：
1995-09-29
影响因子：
6.2
通讯作者：
ROCKE, DM
中科院分区：
文献类型：
--
作者：
JONES, G;WORTBERG, M;ROCKE, DM
酶联免疫吸附测定通常通过在同一微量滴度板上同时运行标准浓度和未知浓度来进行，因为已知标准曲线在每次测定中变化很大。在这里，我们通过实验研究了这种变化的来源和性质，并讨论了通过使用一批板来降低检测成本的可能性，其中只有一个板用于生成校准曲线。我们提出了一种方法，并进行了实证检验。
Enzyme-linked immunosorbent assays are usually performed by running standard and unknown concentrations together on the same microtiter plate, because the standard curve is known to vary considerably from one assay to the next. Here we examine experimentally the sources and nature of this variation, and discuss the possibility of reducing the cost of the assay by using a batch of plates, only one of which is used to generate the calibration curve. We present a method for doing this, and test it empirically.