Comparative genome-wide analysis reveals that Burkholderia contaminans MS14 possesses multiple antimicrobial biosynthesis genes but not major genetic loci required for pathogenesis.
Comparative genome-wide analysis reveals that Burkholderia contaminans MS14 possesses multiple antimicrobial biosynthesis genes but not major genetic loci required for pathogenesis.
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DOI：
10.1002/mbo3.333
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发表时间：
2016-06
期刊：
影响因子：
3.4
通讯作者：
Lu S
中科院分区：
文献类型：
--
作者：
Deng P;Wang X;Baird SM;Showmaker KC;Smith L;Peterson DG;Lu S
污染伯克霍尔德氏菌MS14对动植物病原真菌和细菌具有显著的抗菌活性。MS14菌株生产的抗真菌药物Ocidiofunin在生物农药和药物方面具有很大的开发潜力。然而，由于植物促生菌和病原菌难以区分，限制了伯克霍尔德氏菌作为生防菌在农业上的应用。对完整的MS14基因组进行了测序和分析，以找出它包含哪些有益和毒力相关的基因。此外，还分析了污染伯克霍尔德氏菌MS14与其他17种伯克霍尔德菌的亲缘关系。为了研究MS14‘S的潜在毒力，比较了MS14和其他伯克霍尔德氏菌基因组中与抗生素产生、抗生素耐药性和毒力相关的基因区域。对污染芽孢杆菌MS14的基因组进行了测序和注释。基因组分析揭示了抗菌剂生物合成的多个基因组的存在，这有助于其抗菌活性。BLAST结果表明，MS14基因组含有大量独特的区域。根据平均核苷酸同源性数据，MS14与另一株促进植物生长的Burkholderia菌株B.lata 383有密切的亲缘关系。此外，根据系统发育分析，从土壤中分离的植物促生长物种和哺乳动物致病物种分别聚在一起。MS14具有从基因组中鉴定的多个抗菌活性相关基因，但它缺乏在病原菌中发现的关键毒力相关基因位点。此外，与非植物生长促生型伯克霍尔德氏菌相比，植物生长促生型伯克霍尔德氏菌在其基因组中具有一个或多个抗微生物生物合成基因。另一方面，致病菌种具有非致病伯克霍尔德氏菌所没有的多个毒力相关基因座。MS14基因组和伯克霍尔德氏菌的基因组显示出相当大的多样性。在植物生长促进型伯克霍尔德氏菌的基因组中发现了多种抗菌剂生物合成基因。此外，与非致病性伯克霍尔德氏菌相比，致病性伯克霍尔德氏菌具有更具特征的基因座同源性，这些基因座与致病性和对动植物的致病性有关。
Burkholderia contaminans MS14 shows significant antimicrobial activities against plant and animal pathogenic fungi and bacteria. The antifungal agent occidiofungin produced by MS14 has great potential for development of biopesticides and pharmaceutical drugs. However, the use of Burkholderia species as biocontrol agent in agriculture is restricted due to the difficulties in distinguishing between plant growth‐promoting bacteria and the pathogenic bacteria. The complete MS14 genome was sequenced and analyzed to find what beneficial and virulence‐related genes it harbors. The phylogenetic relatedness of B. contaminans MS14 and other 17 Burkholderia species was also analyzed. To research MS14′s potential virulence, the gene regions related to the antibiotic production, antibiotic resistance, and virulence were compared between MS14 and other Burkholderia genomes. The genome of B. contaminans MS14 was sequenced and annotated. The genomic analyses reveal the presence of multiple gene sets for antimicrobial biosynthesis, which contribute to its antimicrobial activities. BLAST results indicate that the MS14 genome harbors a large number of unique regions. MS14 is closely related to another plant growth‐promoting Burkholderia strain B. lata 383 according to the average nucleotide identity data. Moreover, according to the phylogenetic analysis, plant growth‐promoting species isolated from soils and mammalian pathogenic species are clustered together, respectively. MS14 has multiple antimicrobial activity‐related genes identified from the genome, but it lacks key virulence‐related gene loci found in the pathogenic strains. Additionally, plant growth‐promoting Burkholderia species have one or more antimicrobial biosynthesis genes in their genomes as compared with nonplant growth‐promoting soil‐isolated Burkholderia species. On the other hand, pathogenic species harbor multiple virulence‐associated gene loci that are not present in nonpathogenic Burkholderia species. The MS14 genome as well as Burkholderia species genome show considerable diversity. Multiple antimicrobial agent biosynthesis genes were identified in the genome of plant growth‐promoting species of Burkholderia. In addition, by comparing to nonpathogenic Burkholderia species, pathogenic Burkholderia species have more characterized homologs of the gene loci known to contribute to pathogenicity and virulence to plant and animals.