Evaluation of Phenolic Antioxidant Capacity in Grains of Modern and Old Durum Wheat Genotypes by the Novel QUENCHERABTS Approach
Evaluation of Phenolic Antioxidant Capacity in Grains of Modern and Old Durum Wheat Genotypes by the Novel QUENCHERABTS Approach
复制标题
DOI：
10.1007/s11130-015-0483-8
复制
发表时间：
2015-06-01
影响因子：
4
通讯作者：
Pastore, Donato
中科院分区：
文献类型：
--
作者：
Laus, Maura N.;Di Benedetto, Nilde A.;Pastore, Donato
用于抗氧化能力（AC）测定的猝灭剂（ABTS）（快速、简单、新颖、廉价和可再现）方法基于2，2 '-连氮基-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基阳离子与细小固体食品颗粒的直接反应。因此，它可能类似于食物或人类胃肠道特征中的抗氧化作用。本文采用QUENCHER方法研究了硬粒小麦（Triticum durum Desf.）晶粒首先，评估哪种抗氧化剂决定淬灭反应。这已经进行了比较AC测量淬火（ABTS）和经典的TEAC（ABTS）（Trolox等效抗氧化能力）在四个不同的提取物从全粉的10个硬粒小麦品种含有：亲脂性，亲水性，不溶性结合酚（IBP）和游离可溶性酚（FSP）化合物。QUENCHER（ABTS）数据与水可提取抗氧化剂的AC无关，与亲脂性抗氧化剂的AC弱相关（r = 0.405，P < 0.05）;相反，QUENCHER（ABTS）反应主要与IBP的AC相关（r = 0.907，P < 0.001），与FSP提取物的AC相关性较小（r = 0.747，P < 0.001）。此外，与IBP和FSP的酚类含量也存在相关性（分别为r = 0.760，P < 0.001和r = 0.522，P < 0.01），从而证实了QUENCHER（ABTS）测定主要评估IBP所致AC。因此，在第一次筛选研究中使用该测定来比较在意大利栽培了世纪的36个基因型/地方品种的生物活性IBP的AC。有趣的是，现代和旧基因型之间没有相关的AC差异被发现，从而表明，一个世纪的植物育种并没有降低硬粒小麦中苯酚依赖的健康潜力。
The QUENCHER(ABTS) (QUick, Easy, New, CHEap and Reproducible) approach for antioxidant capacity (AC) determination is based on the direct reaction of 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation with fine solid food particles. So, it may resemble the antioxidant action in foods or in human gastrointestinal trait. Here, the QUENCHER approach was used to study AC of durum wheat (Triticum durum Desf.) grains. Firstly, it was assessed which kind of antioxidants determines QUENCHER response. This has been performed by comparing AC measured by QUENCHER(ABTS) and that measured by classical TEAC(ABTS) (Trolox equivalent antioxidant capacity) in four different extracts from whole flour of 10 durum wheat varieties containing: lipophilic, hydrophilic, insoluble-bound phenolic (IBP) and free-soluble phenolic (FSP) compounds. QUENCHER(ABTS) data were unrelated to AC of water-extractable antioxidants and weakly correlated (r = 0.405, P < 0.05) to AC of the lipophilic ones; on the contrary, QUENCHER(ABTS) response was mainly related to AC of IBP (r = 0.907, P < 0.001) and to a lesser extent of FSP extracts (r = 0.747, P < 0.001). Consistently, correlation was also found with the phenolic content of IBP and FSP (r = 0.760, P < 0.001 and r = 0.522, P < 0.01, respectively), thus confirming that QUENCHER(ABTS) assay mainly assesses AC due to IBP. So, this assay was used in a first screening study to compare AC of bioactive IBP of thirty-six genotypes/landraces covering a century of cultivation in Italy. Interestingly, no relevant AC difference between modern and old genotypes was found, thus suggesting that a century of plant breeding did not decrease phenol-dependent health potential in durum wheat.