Enzyme-Mediated Alleviation of Peroxide Toxicity in Self-Oxygenating Biomaterials.
Enzyme-Mediated Alleviation of Peroxide Toxicity in Self-Oxygenating Biomaterials.
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酶介导减轻自氧化生物材料中的过氧化物毒性。
DOI：
10.1002/adhm.202102697
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发表时间：
2022
影响因子：
10
通讯作者：
Shin,SuRyon
中科院分区：
文献类型：
--
作者：
Willemen,NielsGA;Hassan,Shabir;Gurian,Melvin;Jasso-Salazar,MariaFernanda;Fan,Kai;Wang,Haihang;Becker,Malin;Allijn,IrisE;Bal-Öztürk,Ayça;Leijten,Jeroen;Shin,SuRyon
释放氧气的生物材料可以通过创造具有活性氧水平的环境来促进活体植入物的存活。包封过氧化钙（CPO）的疏水性产氧微粒（HOGMP）最近已用于组织工程，以释放生理相关量的氧气数周。然而，使用CPO产生氧气是通过产生毒性水平的过氧化氢（H2 O2）来介导的。加入抗氧化剂，如过氧化氢酶，可能会降低H2 O2水平。然而，配方中的过氧化氢酶可以最有效地还原H2 O2内产生氧气的生物材料仍然没有探索。在这项研究中，三种不同的过氧化氢酶掺入方法进行了比较，根据他们的能力，以减少过氧化氢水平。具体来说，过氧化氢酶被掺入HOGMP中，或被吸收到HOGMP上，或自由负载到包埋HOGMP的水凝胶中，并与不含过氧化氢酶的对照进行比较。在HOGMP负载的水凝胶中补充游离过氧化氢酶显著降低了H2 O2水平，反映了所有组的更高细胞活力和代谢活性。含有细胞的HOGMP/过氧化氢酶负载的水凝胶前体溶液用作充氧生物墨水，允许在严重缺氧条件下改善打印的构建体的活力。HOGMP与负载过氧化氢酶的水凝胶的组合具有降低产氧组织的过氧化物毒性的潜力。
Oxygen releasing biomaterials can facilitate the survival of living implants by creating environments with a viable oxygen level. Hydrophobic oxygen generating microparticles (HOGMPs) encapsulated calcium peroxide (CPO) have recently been used in tissue engineering to release physiologically relevant amounts of oxygen for several weeks. However, generating oxygen using CPO is mediated via the generation of toxic levels of hydrogen peroxide (H2O2). The incorporation of antioxidants, such as catalases, can potentially reduce H2O2levels. However, the formulation in which catalases can most effectively scavenge H2O2within oxygen generating biomaterials has remained unexplored. In this study, three distinct catalase incorporation methods are compared based on their ability to decrease H2O2levels. Specifically, catalase is incorporated within HOGMPs, or absorbed onto HOGMPs, or freely laden into the hydrogel entrapping HOGMPs and compared with control without catalase. Supplementation of free catalase in an HOGMP‐laden hydrogel significantly decreases H2O2levels reflecting a higher cellular viability and metabolic activity of all the groups. An HOGMP/catalase‐laden hydrogel precursor solution containing cells is used as an oxygenating bioink allowing improved viability of printed constructs under severe hypoxic conditions. The combination of HOGMPs with a catalase‐laden hydrogel has the potential to decrease peroxide toxicity of oxygen generating tissues.