The Balance of Prickle/Spiny-Legs Isoforms Controls the Amount of Coupling between Core and Fat PCP Systems
The Balance of Prickle/Spiny-Legs Isoforms Controls the Amount of Coupling between Core and Fat PCP Systems
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DOI：
10.1016/j.cub.2014.08.005
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发表时间：
2014-09-22
期刊：
影响因子：
9.2
通讯作者：
Juelicher, Frank
中科院分区：
文献类型：
--
作者：
Merkel, Matthias;Sagner, Andreas;Juelicher, Frank
背景资料：保守的脂肪和核心平面细胞极性（PCP）途径共同作用，以指定组织范围内的方向的毛和脊在果蝇翅膀。它们的组分在粘附连接处形成细胞内极化复合物，该复合物耦合相邻细胞的极性并形成全局模式。脂肪和核心PCP系统如何相互作用尚不清楚。一些研究表明，Fat PCP直接定向Core PCP成分形成的模式。其他牵连定向组织重塑指定核心PCP patterns.Results：我们使用遗传学，定量图像分析，物理建模，研究脂肪和核心PCP在翅膀发育过程中的相互作用。我们发现它们的模式在形态发生过程中发生变化，经历了由拮抗性核心PCP蛋白亚型刺和刺腿调控的偶联和解偶联阶段。核心PCP的演变模式是滞后的：早期的核心PCP模式被组织流动修改，然后通过耦合到脂肪PCP，产生引导毛发和脊的顺序模式。我们的数据定量解释了PCP突变体中改变的毛发和嵴极性模式。脂肪和核心PCP之间的过早偶联解释了pk突变体中极性模式的改变。在其他核心PCP突变体中，毛发极性模式直接由脂肪PCP指导。当这两个系统失败，头发仍然在本地对齐，并遵守与veines.Conclusions相关的信号：脂肪和核心PCP系统之间的时间调节耦合，使一个单一的组织发展顺序极性模式，定向不同的形态结构。
Background: The conserved Fat and Core planar cell polarity (PCP) pathways work together to specify tissue-wide orientation of hairs and ridges in the Drosophila wing. Their components form intracellularly polarized complexes at adherens junctions that couple the polarity of adjacent cells and form global patterns. How Fat and Core PCP systems interact is not understood. Some studies suggest that Fat PCP directly orients patterns formed by Core PCP components. Others implicate oriented tissue remodeling in specifying Core PCP patterns.Results: We use genetics, quantitative image analysis, and physical modeling to study Fat and Core PCP interactions during wing development. We show their patterns change during morphogenesis, undergoing phases of coupling and uncoupling that are regulated by antagonistic Core PCP protein isoforms Prickle and Spiny-legs. Evolving patterns of Core PCP are hysteretic: the early Core PCP pattern is modified by tissue flows and then by coupling to Fat PCP, producing sequential patterns that guide hairs and then ridges. Our data quantitatively account for altered hair and ridge polarity patterns in PCP mutants. Premature coupling between Fat and Core PCP explains altered polarity patterns in pk mutants. In other Core PCP mutants; hair polarity patterns are guided directly by Fat PCP. When both systems fail, hairs still align locally and obey signals associated with veins.Conclusions: Temporally regulated coupling between the Fat and Core PCP systems enables a single tissue to develop sequential polarity patterns that orient distinct morphological structures.