Whole transcriptome data of primary human NK cells under hypoxia and interleukin 15 priming: A 2x2 factorial design experiment
Whole transcriptome data of primary human NK cells under hypoxia and interleukin 15 priming: A 2x2 factorial design experiment
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DOI：
10.1016/j.dib.2017.07.018
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发表时间：
2017-10-01
期刊：
影响因子：
1.2
通讯作者：
Lindner, Holger A.
中科院分区：
文献类型：
--
作者：
Figueiredo, Ana Sofia;Killian, Doreen;Lindner, Holger A.
自然杀伤 (NK) 细胞介导针对癌症和细胞内感染的先天免疫，通常在缺氧环境中发挥作用。我们采用 2x2 因子设计进行了微阵列实验，以分析人类 NK 细胞中的基因表达（Velasquez 等人，2016）[l]。在本实验中，来自 5 名健康志愿者的 NK 细胞在低氧或常氧培养条件下用生存因子和炎症细胞因子白细胞介素 15 (IL-15) 引发或不引发 6 小时（总共 20 个样本）。在这里，我们提供了有关控制细胞所经历的实际 O-2 分压 (pO(2)) 的培养设置以及所使用的 RNA 提取程序的详细信息，我们从商业旋转柱协议中优化了该程序以获得高浓度的总 RNA。我们提出了标准化微阵列数据的质量控制分析，以及差异调节基因的概述。这些数据提供了有关 NK 细胞在生理相关低氧条件下对免疫刺激的转录反应的见解。该数据集存放在基因表达综合数据库（登录号 GSE70214）中。 (C) 2017 年作者。由爱思唯尔公司出版
Natural Killer (NK) cells mediate innate immunity against cancer and intracellular infection, at that, operating in often oxygen deprived environments. We performed a microarray experiment with a 2x2 factorial design to profile gene expression in human NK cells (Velasquez et al., 2016) [l]. In this experiment, NK cells from 5 healthy volunteers were primed or not for 6 h with the survival factor and inflammatory cytokine interleukin 15 (IL-15) under hypoxic or normoxic culture conditions (20 samples in total). Here, we provide details on the culture setup that govern the actual O-2 partial pressure (pO(2)) experienced by the cells, as well as on the RNA extraction procedure used, which we optimized from commercial spin column protocols to obtain highly concentrated total RNA. We present a quality control analysis of the normalized microarray data, as well as overviews for differentially regulated genes. These data provide insights into NK cell transcriptional responses to immune stimulation under physiologically relevant low oxygen conditions. This dataset is deposited in the Gene Expression Omnibus database (accession number GSE70214). (C) 2017 The Authors. Published by Elsevier Inc.