A reporter transgene based on a human keratin 6 gene promoter is specifically expressed in the periderm of mouse embryos
A reporter transgene based on a human keratin 6 gene promoter is specifically expressed in the periderm of mouse embryos
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DOI：
10.1016/s0925-4773(00)00489-5
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发表时间：
2001-01-01
影响因子：
2.6
通讯作者：
Coulombe, PA
中科院分区：
文献类型：
--
作者：
Mazzalupo, S;Coulombe, PA
我们报告的lacZ报告基因融合到人角蛋白6a基因的启动子区的发育调控。在小鼠胚胎中，转基因在周皮（胚胎表皮的最外层）中表达，内源性角蛋白6 α和β基因也是如此。一个子集的牙周膜细胞，定位于暂时的上皮融合，已知含有角蛋白6蛋白，我们发现，这些细胞也港LacZ酶活性。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
We report the developmental regulation of a lacZ reporter transgene fused to the promoter region of the human keratin 6a gene. In mouse embryos, the transgene is expressed in the periderm (the outermost layer of embryonic epidermis), as are the endogenous keratin 6 alpha and beta genes. A subset of periderm cells, localized to temporary epithelial fusions, is known to contain keratin 6 protein, and we find that these cells also harbor LacZ enzymatic activity. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.