Insulin binding to human B lymphoblasts is a function of HLA haplotype.
Insulin binding to human B lymphoblasts is a function of HLA haplotype.
复制标题
胰岛素与人 B 淋巴母细胞的结合是 HLA 单倍型的功能。
DOI：
10.1073/pnas.84.5.1351
复制
发表时间：
1987
影响因子：
11.1
通讯作者：
Edidin,M
中科院分区：
文献类型：
--
作者：
Kittur,D;Shimizu,Y;DeMars,R;Edidin,M
各种遗传和生物化学证据表明，主要组织相容性复合体（MHC）单倍型和几种类型的细胞表面受体，包括表皮生长因子和胰岛素受体之间的关联。我们报告了人类I类MHC抗原、HLA抗原和人类B淋巴母细胞上特异性胰岛素结合位点之间存在这种关联的证据。我们已经测量了胰岛素与HLA杂合的EB病毒转化的B细胞系LCL 721细胞的结合，以及与HLA复合物全部或部分缺失的衍生突变体的结合。在表达抗原HLA-B5和其它HLA抗原的突变体和仅表达HLA-C的突变体中，胰岛素结合位点的亲和力Ka约为10（8）M-1。HLA-A1; HLA-A1，B8; HLA-A2，C;和HLA无效突变体（不表达任何HLA抗原）以约10（9）M-1的亲和力将胰岛素结合至位点。
A variety of genetic and biochemical evidence points to an association between major histocompatibility complex (MHC) haplotype and several types of cell surface receptors including epidermal growth factor and insulin receptors. We report evidence for such associations between human class I MHC antigens, HLA antigens, and specific insulin binding sites on human B lymphoblasts. We have measured insulin binding to cells of an HLA-heterozygous, Epstein-Barr virus-transformed B-cell line, LCL 721, and to derivative mutants from which all or part of the HLA complex had been deleted. The affinity, Ka, of insulin binding sites is approximately 10(8) M-1 in mutants expressing antigen HLA-B5 together with other HLA antigens and in mutants expressing only HLA-C. HLA-A1; HLA-A1,B8; HLA-A2,C; and HLA null mutants (not expressing any HLA antigens) bind insulin to sites with an affinity of approximately 10(9) M-1.