Transmembrane adenylyl cyclase regulates amphibian sperm motility through protein kinase A activation
Transmembrane adenylyl cyclase regulates amphibian sperm motility through protein kinase A activation
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DOI：
10.1016/j.ydbio.2010.11.019
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发表时间：
2011-02-01
影响因子：
2.7
通讯作者：
Arranz, Silvia E.
中科院分区：
文献类型：
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作者：
O'Brien, Emma D.;Krapf, Dario;Arranz, Silvia E.
精子活力对于实现受精至关重要。在两栖动物等体外受精动物中，精子以静止状态储存在睾丸中。低渗受精介质的精子生成触发精子活力的激活。蟾蜍精子在人工精浆（ASP）中不动，但在稀释后获得原位鞭毛跳动。除了低渗透压对精子活力激活的影响外，我们还报告说，卵果冻层（EW）的扩散因子调节运动模式，从原位运动转变为渐进运动。两栖动物精子活力激活所涉及的信号转导途径大多是未知的。在本研究中，我们展示了低渗透压触发的运动激活与蛋白激酶 A (PKA) 激活之间的相关性。此外，这是第一项提供有力证据的研究，证明跨膜腺苷酸环化酶 (tmAC) 通过 PKA 激活调节两栖动物精子活力。 (C) 2010 Elsevier Inc. 保留所有权利。
Sperm motility is essential for achieving fertilization. In animals with external fertilization as amphibians, spermatozoa are stored in a quiescent state in the testis. Spermiation to hypotonic fertilization media triggers activation of sperm motility. Bufo arenarum sperm are immotile in artificial seminal plasma (ASP) but acquire in situ flagellar beating upon dilution. In addition to the effect of low osmolarity on sperm motility activation, we report that diffusible factors of the egg jelly coat (EW) regulate motility patterns, switching from in situ to progressive movement. The signal transduction pathway involved in amphibian sperm motility activation is mostly unknown. In the present study, we show a correlation between motility activation triggered by low osmotic pressure and activation of protein kinase A (PKA). Moreover, this is the first study to present strong evidences that point toward a role of a transmembrane adenyl-cyclase (tmAC) in the regulation of amphibian sperm motility through PKA activation. (C) 2010 Elsevier Inc. All rights reserved.