Identification and differential expression of microRNAs during metamorphosis of the Japanese flounder (Paralichthys olivaceus).
Identification and differential expression of microRNAs during metamorphosis of the Japanese flounder (Paralichthys olivaceus).
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DOI：
10.1371/journal.pone.0022957
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Fu Y;Shi Z;Wu M;Zhang J;Jia L;Chen X
microRNA（miRNAs）是一类长度为20-25个核苷酸的内源性小分子非编码RNA，在多种生物学过程中发挥着重要作用。日本比目鱼在早期发育过程中经历了戏剧性的蜕变。变态的特征是从两侧对称的身体形态转变为不对称的身体形态，伴随着广泛的器官形态和生理重塑。到目前为止，在鱼类中发现的miRNAs很少，而在比目鱼中发现的miRNAs的报道也很少。采用Solexa测序技术对牙鲆变态期小RNA文库进行高通量测序。随后，将这些测序数据与后生动物已知的miRNA进行比对，我们表征了来自小RNA文库的140个保守的miRNA和57个miRNA：miRNA* 对。在这57个miRNA：miRNA* 对中，从基因组DNA扩增出20个比目鱼miRNA前体。我们还证明了在动物进化过程中日本比目鱼miRNAs和miRNAs * 的进化保守性。利用miRNA微阵列技术，我们鉴定了66个在两个变质阶段（孵化后17天和29天）的日本比目鱼差异表达的miRNA。结果表明，miRNAs可能在调控牙鲆变态过程中的基因表达中起关键作用。我们发现了大量的miRNA在比目鱼变态，其中一些差异表达在两个不同的变态阶段。该研究为进一步了解miRNA在比目鱼变态调控中的作用提供了机会，也为进一步研究比目鱼变态机制提供了线索。
MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs of 20–25 nucleotides that play a key role in diverse biological processes. Japanese flounder undergo dramatic metamorphosis in their early development. The metamorphosis is characterized by morphological transformation from a bilaterally symmetrical to an asymmetrical body shape concomitant with extensive morphological and physiological remodeling of organs. So far, only a few miRNAs have been identified in fish and there are very few reports about the Japanese flounder miRNA. Solexa sequencing technology was used to perform high throughput sequencing of the small RNA library from the metamorphic period of Japanese flounder. Subsequently, aligning these sequencing data with metazoan known miRNAs, we characterized 140 conserved miRNAs and 57 miRNA: miRNA* pairs from the small RNA library. Among these 57 miRNA: miRNA* pairs, twenty flounder miRNA precursors were amplified from genomic DNA. We also demonstrated evolutionary conservation of Japanese flounder miRNAs and miRNA* in the animal evolution process. Using miRNA microarrays, we identified 66 differentially expressed miRNAs at two metamorphic stages (17 and 29 days post hatching) of Japanese flounder. The results show that miRNAs might play a key role in regulating gene expression during Japanese flounder metamorphosis. We identified a large number of miRNAs during flounder metamorphosis, some of which are differentially expressed at two different metamorphic stages. The study provides an opportunity for further understanding of miRNA function in the regulation of flounder metamorphosis and gives us clues for further studies of the mechanisms of metamorphosis in Japanese flounder.
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通过 Solexa 测序鉴定和表征新型文昌鱼 microRNA
影响因子：
12.3
作者：
Chen X;Li Q;Wang J;Guo X;Jiang X;Ren Z;Weng C;Sun G;Wang X;Liu Y;Ma L;Chen JY;Wang J;Zen K;Zhang J;Zhang CY
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通讯作者：
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DOI：
10.1073/pnas.0907391106
发表时间：
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影响因子：
11.1
作者：
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通讯作者：
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影响因子：
64.8
作者：
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