In vitro induction of secondary xylem-like tracheary elements in calli of hybrid poplar (Populus sieboldii × P. grandidentata)
In vitro induction of secondary xylem-like tracheary elements in calli of hybrid poplar (Populus sieboldii × P. grandidentata)
复制标题
杂交杨愈伤组织中次生木质部样气管元件的体外诱导（西氏杨×大齿杨）
作者：
Yamagishi;Y.;Yoshimoto;J.;Uchiyama;H.;Nabeshima;E.;Nakaba;S.;Watanabe;U.;Funada;R.
以杂交白杨（Populus sieboldii×P. grandidentata）在缺乏生长素但含有1 μM芸苔素的培养基上培养10天后，部分分化细胞形成宽的细胞壁区和具缘纹孔，这是次生木质部导管分子的典型特征。其他分化细胞类似初生木质部的导管分子，细胞壁螺旋状或网状加厚。在愈伤组织中发育的导管分子在细胞簇内形成。该诱导体系为研究次生木质部细胞的分化机制提供了一个新的模型。
The formation of tracheary elements was induced in calli derived from petioles of hybrid poplar (Populus sieboldii×P. grandidentata) after 10 days of culture on medium that lacked auxin but contained 1 μM brassinolide. Some differentiated cells formed broad regions of cell walls and bordered pits, which are typical features of tracheary elements of secondary xylem. Other differentiated cells resembled tracheary elements of primary xylem, with spiral or reticulate thickening of cell walls. The tracheary elements that developed in calli were formed within cell clusters. This induction system provides a new model for studies of the mechanism of differentiation of secondary xylem cells in vitro.
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影响因子：
4
作者：
Möller, R;Koch, G;Schmitt, U
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2.8
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4.3
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