Overexpression of Pim-1 during progression of prostatic adenocarcinoma
Overexpression of Pim-1 during progression of prostatic adenocarcinoma
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DOI：
10.1136/jcp.2005.027672
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发表时间：
2006-03-01
影响因子：
3.4
通讯作者：
Cheng, L
中科院分区：
文献类型：
--
作者：
Cibull, TL;Jones, TD;Cheng, L
目的：Pim-1是一种丝氨酸/苏氨酸激酶，已被证明在许多人类癌症，如血液淋巴样恶性肿瘤的发展中发挥不可或缺的作用。最近，有证据表明Pim-1在前列腺癌发生中是重要的。为了进一步了解Pim-1在前列腺癌中的作用，我们研究了Pim-1在正常前列腺组织、癌前病变组织和前列腺癌组织中的表达。在每种情况下，Pim-1染色在良性前列腺上皮，高级别前列腺上皮内瘤（PIN）和前列腺腺癌进行了评价。结果：Pim-1在腺癌、高级别PIN和良性腺体中阳性表达率分别为100%、100%和52%。良性上皮中染色的细胞数（平均34%）远低于高级别PIN（平均80%; p < 0.0001）或腺癌（平均84%; p < 0.0001）。在高级别PIN和腺癌之间，Pim-1阳性染色的细胞百分比没有显著差异（p=0.34）。Pim-1在良性前列腺上皮中的表达强度显著低于PIN和腺癌（p < 0.001）。Pim-1表达水平与Gleason评分、患者年龄、肿瘤分期、淋巴结转移、神经浸润、血管浸润、手术切缘状态、前列腺外扩展或精囊浸润之间无统计学显著相关性。这一发现表明，Pim-1的上调可能在前列腺肿瘤中发挥作用。
Aims: Pim-1 is a serine/threonine kinase that has been shown to play an integral role in the development of a number of human cancers, such as haematolymphoid malignancies. Recently, evidence has shown Pim-1 to be important in prostatic carcinogenesis. In order to further our understanding of its role in prostate cancer, we investigated Pim-1 expression in normal, premalignant, and malignant prostate tissue.Methods: Using immunohistochemistry, Pim-1 expression was analysed in prostate tissue from 120 radical prostatectomy specimens. In each case, Pim-1 staining was evaluated in benign prostatic epithelium, high grade prostatic intraepithelial neoplasia (PIN), and prostatic adenocarcinoma. The number of positively staining cells was estimated, and the intensity of staining was scored on a scale of 0 to 3+.Results: Pim-1 immunoreactivity was identified in 120 cases (100%) of adenocarcinoma, 120 cases (100%) of high grade PIN, and 62 cases (52%) of benign glands. The number of cells staining in benign epithelium ( mean 34%) was much lower than that in high grade PIN (mean 80%; p < 0.0001) or adenocarcinoma (mean, 84%; p < 0.0001). There was no significant difference between high grade PIN and adenocarcinoma in the percentage of cells staining positively for Pim-1 (p=0.34). The staining intensity for Pim-1 was significantly lower in benign prostatic epithelium than in PIN and adenocarcinoma (p < 0.001). There was no statistically significant correlation between the level of Pim-1 expression and Gleason score, patient age, tumour stage, lymph node metastasis, perineural invasion, vascular invasion, surgical margin status, extraprostatic extension, or seminal vesicle invasion.Conclusions: Pim-1 expression is elevated in PIN and prostatic adenocarcinoma compared with benign prostatic epithelium. This finding suggests that upregulation of Pim-1 may play a role in prostatic neoplasia.