Abscisic Acid Negatively Modulates Heat Tolerance in Rolled Leaf Rice by Increasing Leaf Temperature and Regulating Energy Homeostasis
Abscisic Acid Negatively Modulates Heat Tolerance in Rolled Leaf Rice by Increasing Leaf Temperature and Regulating Energy Homeostasis
复制标题
脱落酸通过提高叶温和调节能量稳态来负调节卷叶米的耐热性
DOI：
10.1186/s12284-020-00379-3
复制
发表时间：
2020-03-13
期刊：
影响因子：
5.5
通讯作者：
Fu, Guanfu
中科院分区：
文献类型：
--
作者：
Li, Guangyan;Zhang, Caixia;Fu, Guanfu
背景脱甲酸（ABA）充当植物中针对非生物应激的信号激素，但其在热应激下的能量稳态中的功能尚不清楚。结果将两种水稻基因型，即扁平叶子的Nipponbare（野生型，WT）及其突变体高温易感性（HTS）植物，其半卷叶，遭受热应激。我们发现，叶片中的组织温度，呼吸速率，ABA和H2O2含量明显更高，而HTS中的蒸腾速率和气孔电导率低于WT植物。此外，在热应激下，在WT中检测到Hsp71.1和Hsp24.1的表达增加，以及在WT中检测到的碳水化合物含量，ATP，NAD（H）和干物质重量的增加。更重要的是，外源ABA显着降低了HTS植物的热耐受性，但明显增强了WT植物的耐热性。在热应激下，ABA增强了WT植物中碳水化合物，ATP，NAD（H）和热休克蛋白的增加，而HTS植物中这些增加的增加。结论是，得出结论，ABA是通过调节能量稳态的HTS植物中耐热植物的负调节剂。
BackgroundAbscisic acid (ABA) acts as a signaling hormone in plants against abiotic stress, but its function in energy homeostasis under heat stress is unclear.ResultsTwo rice genotypes, Nipponbare (wild-type, WT) with flat leaves and its mutanthigh temperature susceptibility(hts) plant with semi-rolled leaves, were subjected to heat stress. We found significantly higher tissue temperature, respiration rate, and ABA and H2O2contents in leaves as well as a lower transpiration rate and stomatal conductance inhtsthan WT plants. Additionally, increased expression ofHSP71.1andHSP24.1as well as greater increases in carbohydrate content, ATP, NAD (H), and dry matter weight, were detected in WT thanhtsplants under heat stress. More importantly, exogenous ABA significantly decreased heat tolerance ofhtsplants, but clearly enhanced heat resistance of WT plants. The increases in carbohydrates, ATP, NAD (H), and heat shock proteins in WT plants were enhanced by ABA under heat stress, whereas these increases were reduced inhtsplants.ConclusionIt was concluded that ABA is a negative regulator of heat tolerance inhtsplants with semi-rolled leaves by modulating energy homeostasis.