The response of large and small luteal cells from the pregnant rat to substrates and secretagogues.
The response of large and small luteal cells from the pregnant rat to substrates and secretagogues.
复制标题
怀孕大鼠的大黄体细胞和小黄体细胞对底物和促分泌素的反应。
DOI：
10.1095/biolreprod41.6.1123
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发表时间：
1989
影响因子：
3.6
通讯作者：
Sridaran,R
中科院分区：
文献类型：
--
作者：
Smith,CJ;Greer,TB;Banks,TW;Sridaran,R
在酶解离后，通过淘洗分离来自第8天妊娠大鼠的大（> 22 µm）和小（12-21 µm）黄体细胞。将等份细胞在37°C下在单独的培养基199（对照）或含有0.5 mM或5 mM二丁酰环腺苷3′，5′-单磷酸（cAMP）、1、10、100或1000 ng/ml剂量的大鼠促黄体激素（LH）、10 µg/ml 25-OH-胆固醇或10 ng/ml睾酮的培养基中孵育4 h。孕酮、睾酮和雌二醇的产生用放射免疫法测定。当用LH（1 μg/ml）或双丁酰cAMP（5 mM）刺激时，两种细胞类型显示出相似的雌二醇合成增加;然而，大的黄体细胞芳香化外源性睾酮，而小的黄体细胞则没有。与小细胞相比，大黄体细胞在较低剂量的二丁酰cAMP（0.5 mM）和LH（10 ng/ml）下产生的孕酮量增加，小细胞需要5 mM二丁酰cAMP或1 µg/ml LH才能产生最小刺激。二丁酰cAMP（5 mM）也导致小黄体细胞释放睾酮的增加。25-OH-胆固醇增强了两种细胞类型中的孕酮合成。这些结果表明，这两种类型的细胞在功能上不同，在怀孕期间类固醇的生成，大黄体细胞似乎是孕激素和雌二醇生产的主要网站在这个阶段的怀孕。
Large (> 22 µm) and small (12–21 µm) luteal cells from Day 8 pregnant rats were separated by elutriation after enzyme dissociation. Aliquots of cells were incubated for 4 h at 37°C in Medium 199 alone (control) or with medium containing dibutyryl cyclic adenosine 3′, 5′-monophosphate (cAMP) at 0.5 mM or 5 mM; rat luteinizing hormone (LH) at doses of 1, 10, 100, or 1000 ng/ml; 10 µg/ml 25-OH-cholesterol; or 10 ng/ml testosterone. Production of progesterone, testosterone, and estradiol was measured by radioimmunoassay. Both cell types showed a similar increase in estradiol synthesis when stimulated with LH (1 µg/ml) or dibutyryl cAMP (5 mM); however, large luteal cells aromatized exogenous testosterone, whereas small luteal cells did not. Large luteal cells produced increased amounts of progesterone at lower doses of dibutyryl cAMP (0.5 mM) and LH (10 ng/ml), compared to small cells, which required 5 mM dibutyryl cAMP or 1 µg/ml LH for minimal stimulation. Dibutyryl cAMP (5 mM) also resulted in an increase of testosterone release from small luteal cells. Progesterone synthesis in both cell types was enhanced by 25-OH-cholesterol. These results suggest that the two cell types differ functionally with respect to steroidogenesis during pregnancy, and that the large luteal cells appear to be the primary site of progesterone and estradiol production at this stage of pregnancy.