Development and regional expression of beta nerve growth factor messenger RNA and protein in the rat central nervous system.
Development and regional expression of beta nerve growth factor messenger RNA and protein in the rat central nervous system.
复制标题
大鼠中枢神经系统中 β 神经生长因子信使 RNA 和蛋白质的发育和区域表达。
DOI：
--
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
H. Persson
中科院分区：
文献类型：
--
作者：
S. Whittemore;T. Ebendal;L. Lärkfors;L. Olson;A. Seiger;I. Strömberg;H. Persson
记录了大鼠中枢神经系统中神经生长因子（NGF）mRNA和蛋白的存在。印迹杂交分析显示，丰富的神经生长因子mRNA在海马，大脑皮层，嗅球。酶免疫测定证实了海马和大脑皮层中神经生长因子样蛋白的显着水平。从这些地区的神经生长因子样免疫亲和纯化蛋白的生物测定是从神经生长因子生理上无法区分。免疫组化显示，在成人大脑中，神经生长因子样反应的广泛分布，优先在纤维束。NGFmRNA的积累开始于出生时，成人水平达到出生后3周。酶免疫法检测胚胎大鼠脑中存在的神经生长因子样蛋白。生后3周时，神经生长因子样蛋白水平达到最高。此外，可能存在不同的胎儿形式的NGF。
The presence of nerve growth factor (NGF) mRNA and protein in the rat central nervous system is documented. Blot-hybridization analysis showed an abundance of NGF mRNA in the hippocampus, cerebral cortex, and olfactory bulb. Enzyme immunoassay confirmed significant levels of a NGF-like protein in the hippocampus and cerebral cortex. Bioassay of a NGF-like immunoaffinity-purified protein from these regions was physiologically indistinguishable from NGF. Immunohistochemistry revealed a widespread distribution of NGF-like reactivity in the adult brain, preferentially in fiber tracts. NGF mRNA accumulation began at birth, with adult levels reached 3 weeks postnatally. Enzyme immunoassay detected the presence of a NGF-like protein in the embryonic rat brain. Postnatally, the level of NGF-like protein reached a maximum at 3 weeks. Additionally, a distinct fetal form of NGF may exist.