Proteomic identification of a two-component regulatory system in Pseudoalteromonas haloplanktis TAC125
Proteomic identification of a two-component regulatory system in Pseudoalteromonas haloplanktis TAC125
复制标题
盐浮游假交替单胞菌 TAC125 中双组分调控系统的蛋白质组学鉴定
作者：
R. Papa;Susanne Glagla;Antoine Danchin;T. Schweder;G. Marino;A. Duilio
微生物利用不同碳水化合物作为能源的能力反映了这些底物在其栖息地的可用性。对碳水化合物使用所涉及的蛋白质的研究，与其表达的分析并行，可能会提供有关微生物与其生态系统之间相互作用的信息。我们分析了南极海洋细菌 Pseudoalteromonas haloplanktis TAC125 在存在和不存在不同碳源的情况下的生长行为。当向生长培养基中添加l-苹果酸时，检测到光密度显着增加。通过蛋白质组分析实验鉴定了在 L-苹果酸存在下不同表达的细菌蛋白质。根据其相对增加，选择六种蛋白质进行进一步分析。其中，假定的外膜孔蛋白的表达被证明受到l-苹果酸的强烈诱导。在孔蛋白基因的上游区域发现了功能活跃的双组分调节系统的存在，很可能由 l-苹果酸控制。然后构建了非功能性基因组孔蛋白突变体，显示该蛋白直接参与 L-苹果酸的摄取。据我们所知，迄今为止，在假交替单胞菌中从未报道过这种调控系统的出现，这可能构成开发有效的诱导冷表达系统的关键一步。
The capability of microorganisms to utilize different carbohydrates as energy source reflects the availability of these substrates in their habitat. Investigation of the proteins involved in carbohydrate usage, in parallel with analysis of their expression, is then likely to provide information on the interaction between microorganisms and their ecosystem. We analysed the growth behaviour of the marine Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 in the presence and in the absence of different carbon source. A marked increase in the optical density was detected when l-malate was added to the growth medium. Bacterial proteins differently expressed in the presence of l-malate were identified by proteomic profiling experiments. On the basis of their relative increase, six proteins were selected for further analyses. Among these, the expression of a putative outer membrane porin was demonstrated to be heavily induced by l-malate. The presence of a functionally active two-component regulatory system very likely controlled by l-malate was found in the upstream region of the porin gene. A non functional genomic porin mutant was then constructed showing a direct involvement of the protein in the uptake of l-malate. To the best of our knowledge, the occurrence of such a regulatory system has never been reported in Pseudoalteromonads so far and might constitute a key step in the development of an effective inducible cold expression system.
在呼吸缺陷宿主细胞中生长的普瓦泽基立克次体中 gltA 和 TLC 基因的转录调控。
DOI：
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发表时间：
1997
期刊：
Acta virologica.
影响因子：
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作者：
Cai,J;Winkler,HH
通讯作者：
Winkler,HH