A two-stage cyclone using microcentrifuge tubes for personal bioaerosol sampling
A two-stage cyclone using microcentrifuge tubes for personal bioaerosol sampling
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DOI：
10.1039/b609083d
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发表时间：
2006-01-01
影响因子：
--
通讯作者：
Chen, Bean T.
中科院分区：
文献类型：
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作者：
Lindsley, William G.;Schmechel, Detlef;Chen, Bean T.
个人气溶胶采样器被广泛用于监测人体暴露于空气中的物质。对于生物气溶胶，使用分子和免疫学技术分析样品的兴趣正在增长。本文介绍了一种个人采样器，使用两级旋风收集生物气溶胶到一次性1.5毫升Eppendorf型微量离心管。样品可以在试管中进行聚合酶链反应（PCR）或免疫测定，并且使用多个阶段通过空气动力学直径分级气溶胶颗粒。使用荧光微球和雾化真菌孢子对采样器进行测试。采样器在2 l min（-1）时的第一和第二级截止直径分别为2.6 μ m和1.6 μ m（几何标准差，GSD = 1.45和1.75），在3.5 l min（-1）时的第一和第二级截止直径分别为1.8 μ m和1 μ m（GSD = 1.42和1.55）。对于空气动力学直径为3.1 μ m或更小的颗粒，在两种流速下的采样器抽吸效率均>= 98%。对于6.2 μ m的颗粒，抽吸效率在2 l min（-1）时为89%，在3.5 l min（-1）时为96%。在3.5 l min（-1）时，采样器在两个微量离心管内收集了92%的雾化杂色曲霉和产黄青霉孢子，在备用过滤器上收集了不到0.4%的孢子。这里给出的设计和技术适用于个人生物气溶胶采样，也可以适用于设计更大的气溶胶采样器，用于长期的大气和室内空气质量采样。
Personal aerosol samplers are widely used to monitor human exposure to airborne materials. For bioaerosols, interest is growing in analyzing samples using molecular and immunological techniques. This paper presents a personal sampler that uses a two-stage cyclone to collect bioaerosols into disposable 1.5 ml Eppendorf-type microcentrifuge tubes. Samples can be processed in the tubes for polymerase chain reaction ( PCR) or immunoassays, and the use of multiple stages fractionates aerosol particles by aerodynamic diameter. The sampler was tested using fluorescent microspheres and aerosolized fungal spores. The sampler had first and second stage cut-off diameters of 2.6 mu m and 1.6 mu m at 2 l min(-1) ( geometric standard deviation, GSD = 1.45 and 1.75), and 1.8 mu m and 1 mu m at 3.5 l min(-1) ( GSD = 1.42 and 1.55). The sampler aspiration efficiency was >= 98% at both flow rates for particles with aerodynamic diameters of 3.1 mu m or less. For 6.2 mu m particles, the aspiration efficiency was 89% at 2 l min(-1) and 96% at 3.5 l min(-1). At 3.5 l min(-1), the sampler collected 92% of aerosolized Aspergillus versicolor and Penicillium chrysogenum spores inside the two microcentrifuge tubes, with less than 0.4% of the spores collecting on the back-up filter. The design and techniques given here are suitable for personal bioaerosol sampling, and could also be adapted to design larger aerosol samplers for longer-term atmospheric and indoor air quality sampling.