Regulation of jasmonate signaling by reversible acetylation of TOPLESS in Arabidopsis
Regulation of jasmonate signaling by reversible acetylation of TOPLESS in Arabidopsis
复制标题
拟南芥中 TOPLESS 可逆乙酰化对茉莉酸信号传导的调节。
DOI：
10.1016/j.molp.2022.06.014
复制
发表时间：
2022-08-04
期刊：
影响因子：
27.5
通讯作者：
Li, Chuanyou
中科院分区：
文献类型：
--
作者：
An, Chunpeng;Deng, Lei;Li, Chuanyou
植物激素茉莉酸(JA)通过编排全基因组转录程序来调节植物的免疫和适应性生长。JA反应基因表达的关键调控因子包括更重要的转录因子MYC2，它在静息状态下受到保守的Groucho/Tup1样辅阻遏子TPL的抑制。然而，TPL介导的MYC2活性的转录抑制以及激素依赖的抑制和去抑制之间的转换的机制仍然是个谜。在这里，我们报道了TPL的可逆乙酰化对TPL活性和JA信号的调节。我们发现组蛋白乙酰转移酶GCN5可以介导TPL乙酰化，从而增强其与新型JAZ相互作用者(NINJA)适配器的相互作用，并促进其募集到MYC2靶启动子，从而促进转录抑制。相反，由His-Tone脱乙酰酶HDA6进行的TPL脱乙酰化减弱了TPL？忍者之间的相互作用，并抑制了TPL向MYC2靶启动子的募集，促进了转录激活。在静息状态下，GCN5和HDA6的相反活性维持TPL乙酰化的动态平衡，促进TPL的转录抑制活性。在JA的诱导下，HDA6的表达被瞬时诱导，导致TPL乙酰化和抑制活性降低，从而激活MYC2靶基因的转录。因此，GCN5？？TPL？HDA6模块主要保持乙酰化TPL的动态平衡，从而决定JA反应基因的转录状态。我们的发现揭示了一种机制，通过这种机制，JA信号中的TPL辅阻遏子活性可以快速和可逆地主动调节。
The plant hormone jasmonate (JA) regulates plant immunity and adaptive growth by orchestrating a genome-wide transcriptional program. Key regulators of JA-responsive gene expression include the mas-ter transcription factor MYC2, which is repressed by the conserved Groucho/Tup1-like corepressor TOPLESS (TPL) in the resting state. However, the mechanisms underlying TPL-mediated transcriptional repression of MYC2 activity and hormone-dependent switching between repression and de-repression remain enigmatic. Here, we report the regulation of TPL activity and JA signaling by reversible acetylation of TPL. We found that the histone acetyltransferase GCN5 could mediate TPL acetylation, which enhances its interaction with the NOVEL-INTERACTOR-OF-JAZ (NINJA) adaptor and promotes its recruitment to MYC2 target promoters, facilitating transcriptional repression. Conversely, TPL deacetylation by the his -tone deacetylase HDA6 weakens TPL???NINJA interaction and inhibits TPL recruitment to MYC2 target pro-moters, facilitating transcriptional activation. In the resting state, the opposing activities of GCN5 and HDA6 maintain TPL acetylation homeostasis, promoting transcriptional repression activity of TPL. In response to JA elicitation, HDA6 expression is transiently induced, resulted in decreased TPL acetylation and repressor activity, thereby transcriptional activation of MYC2 target genes. Thus, the GCN5???TPL???HDA6 module main-tains the homeostasis of acetylated TPL, thereby determining the transcriptional state of JA-responsive genes. Our findings uncovered a mechanism by which the TPL corepressor activity in JA signaling is actively tuned in a rapid and reversible manner.