Natural variation of GhSI7 increases seed index in cotton
Natural variation of GhSI7 increases seed index in cotton
复制标题
GhSI7 的自然变异增加了棉花的种子指数
DOI：
10.1007/s00122-022-04209-6
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发表时间：
2022-09
期刊：
影响因子：
--
通讯作者：
Zhengsheng Zhang
中科院分区：
文献类型：
--
作者：
Xueying Liu;Juan Hou;Li Chen;Qingqing Li;Xiaomei Fang;Jinxia Wang;Yongshui Hao;Peng Yang;Wenwen Wang;Dishen Zhang;Dexin Liu;Kai Guo;Zhonghua Teng;Dajun Liu;Zhengsheng Zhang
关键信息qSI07.1是棉花种子指数的主效QTL，定位于17.45kb区域，候选基因GhSI7在转基因植株中得到验证。产量相关性状种子指数是一个复杂的数量性状，但种子指数的候选基因很少。在这里，一个主要的QTL种子指数qSI07.1被精细定位到17.45 kb的区域，通过连锁分析和替代作图。只有GhSI7，编码转录调节子无菌无瓣，被包含在候选区域。关联分析和遗传分析表明，其内含子中的一个845 bp的缺失是导致种子指数变异的原因。起源分析表明，这种变异是陆地棉所特有的，起源于小种。GhSI7（单倍型2）的过表达显著增加了棉花植株的种子指数和器官大小。本研究结果为选育高种子指数棉花品种提供了诊断标记，并为进一步研究棉花种子形态建成的分子机制奠定了基础。
Key message qSI07.1, a major QTL for seed index in cotton, was fine-mapped to a 17.45-kb region, and the candidate gene GhSI7 was verified in transgenic plants.Improving production to meet human needs is a vital objective in cotton breeding. The yield-related trait seed index is a complex quantitative trait, but few candidate genes for seed index have been characterized. Here, a major QTL for seed index qSI07.1 was fine-mapped to a 17.45-kb region by linkage analysis and substitutional mapping. Only GhSI7, encoding the transcriptional regulator STERILE APETALA, was contained in the candidate region. Association test and genetic analysis indicated that an 845-bp-deletion in its intron was responsible for the seed index variation. Origin analysis revealed that this variation was unique in Gossypium hirsutum and originated from race accessions. Overexpression of GhSI7 (haplotype 2) significantly increased the seed index and organ size in cotton plants. Our findings provided a diagnostic marker for breeding and selecting cotton varieties with high seed index, and laid a foundation for further studies to understand the molecular mechanism of cotton seed morphogenesis.
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作者：
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通讯作者：
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DOI：
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作者：
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