Optical mapping of BAC clones from the human Y chromosome DAZ locus
Optical mapping of BAC clones from the human Y chromosome DAZ locus
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DOI：
10.1101/gr.112100
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发表时间：
2000-09-01
期刊：
影响因子：
7
通讯作者：
Schwartz, DC
中科院分区：
文献类型：
--
作者：
Giacalone, J;Delobette, S;Schwartz, DC
从包含重复元素的复杂排列的基因组区域衍生的克隆的精确定位为DNA测序提出了特殊的问题。光学定位自动化的最新进展使我们能够绘制一组来自人类Y染色体长臂DAZ位点的16个BAC克隆，在这个位点上，整个DAZ基因以及基因拷贝内的亚段都被复制了。使用7种酶的高分辨率光学定位将这些克隆放置在两个组中，代表DAZ基因的四个不同拷贝，并突出了DAZ单个拷贝之间的许多差异。
The accurate mapping of clones derived from genomic regions containing complex arrangements of repeated elements presents special problems for DNA sequencers. Recent advances in the automation of optical mapping have enabled us to map a set of 16 BAC clones derived from the DAZ locus of the human Y chromosome long arm, a locus in which the entire DAZ gene as well as subsections within the gene copies have been duplicated. High-resolution optical mapping employing seven enzymes places these clones into two contigs representing four distinct copies of the DAZ gene and highlights a number of differences between individual copies of DAZ.