Performing IVIM-DWI using the multifunctional nanosystem for the evaluation of the antitumor microcirculation changes
Performing IVIM-DWI using the multifunctional nanosystem for the evaluation of the antitumor microcirculation changes
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使用多功能纳米系统进行 IVIM-DWI 评估抗肿瘤微循环变化
DOI：
10.1007/s10334-019-00814-7
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发表时间：
2020-01-03
影响因子：
2.3
通讯作者：
Luo, Liangping
中科院分区：
文献类型：
--
作者：
Gao, Peng;Liu, Yiyong;Luo, Liangping
目的体素内非相干运动弥散加权成像（IVIM-DWI）的D * 和f值在肿瘤血流灌注中长期疗效监测中存在争议。采用IVIM-DWI技术监测F/A-PLGA@DOX/SPIO纳米系统对非小细胞肺癌（NSCLC）的治疗效果，探讨伪扩散系数（D*）和伪扩散分数（f）在评价其疗效中的价值。第1组（G1）注射生理盐水（对照组）。第2组（G2）和第3组（G3）分别注射DOX和F/A-PLGA@DOX/SPIO。各组分别于治疗前及治疗后3、14、21、28天行IVIM-DWI扫描，采用GE AW 4.5后处理工作站计算D* 和f值。结果3组小鼠D * 值均呈上升趋势，G1组上升幅度最大，G3组最小。相反，所有组的f值在7天内呈下降趋势，其中G3表现出最显著的下降。免疫组化显示，G3组肿瘤血管内皮生长因子（VEGF）阳性表达率和微血管密度（MVD）均显著低于其他各组（P< 0.05）。D * 和f值与CD 31（r= 0.654，P < 0.001;r= 0.712，P < 0.001）和VEGF（r= 0.694，P <0.001;r= 0.664，P < 0.001）呈显著正相关。
ObjectivesThere is a controversy about theD* andfvalues of intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI) for mid- and long-term efficacy monitoring of tumor blood perfusion. To monitor the antitumor efficacy of the F/A-PLGA@DOX/SPIO nanosystem via IVIM-DWI and to explore the value of parameters pseudo-diffusion (D*) and fraction of pseudo-diffusion (f) for evaluating therapeutic effect in non-small cell lung cancer (NSCLC).Materials and methodsThirty-six A549 tumor-bearing mice were divided randomly into three groups (eachn= 12). Group 1 (G1) was injected with saline (the control group). Group 2 (G2) and group 3(G3) were injected with DOX and F/A-PLGA@DOX/SPIO, respectively. Each group underwent IVIM-DWI scanning at baseline and 3, 14, 21, and 28 days after treatment.D* andfvalues were derived using GE AW 4.5 post-processing station. All mice were sacrificed for pathological examination.ResultsTheD* value of all three groups showed an upward trend, with the highest increase in G1 and the lowest in G3. Conversely, thefvalue of all groups trended to decrease within 7 days, of which G3 showed the most significant decline. Immunohistochemical staining revealed that vascular endothelial growth factor (VEGF)-positive staining rate and the microvessel density (MVD) of the tumors in G3 were significantly lower than those of the other groups (P< 0.05). TheD* andfvalues were significantly and positively correlated to CD31 (r= 0.654,P< 0.001;r= 0.712,P< 0.001) and VEGF (r= 0.694,P< 0.001;r= 0.664,P< 0.001).ConclusionIVIM-DWI-derived parametersD* and f are valuable indicators for the evaluation of the antitumor microcirculation changes of multifunctional nanosystem.