Epithelial heterogeneity in the murine thymus: fucose-specific lectins bind medullary epithelial cells.
Epithelial heterogeneity in the murine thymus: fucose-specific lectins bind medullary epithelial cells.
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DOI：
10.4049/jimmunol.134.5.2971
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发表时间：
1985-05
影响因子：
4.4
通讯作者：
A. Farr;S. Anderson
中科院分区：
文献类型：
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作者：
A. Farr;S. Anderson
通过组织化学技术，发现两种对L-岩藻糖具有标称特异性的凝集素，Tetragonolobus purpureas凝集素（TPA）和荆豆凝集素（UEA）特异性标记小鼠胸腺的髓质区。虽然这两种凝集素的结合仅限于胸腺的髓质区，但每种染色模式都是独特的。结合UEA的细胞在整个髓质形成网状网络，而结合TPA的细胞以单细胞或小细胞团的形式出现，类似于Hassall小体。结合任一凝集素的细胞根据超微结构特征（张力丝、桥粒和透明角质体）被鉴定为上皮细胞，并且类似于先前描述的Ia+髓质上皮细胞。观察到UEA结合的年龄相关性下降，而TPA标记保持不变。UEA和TPA和这两种凝集素的特异性的报告获得的标记模式的基础上，它表明，大多数检测到的岩藻糖是与1型碳水化合物链。
By means of histochemical techniques, two lectins with nominal specificity for L-fucose, Tetragonolobus purpureas agglutinin (TPA), and Ulex europeus agglutinin (UEA) were found to specifically label the medullary area of murine thymuses. Although the binding of both lectins was restricted to the medullary area of the thymus, each staining pattern was unique. Cells binding UEA formed a reticular network throughout the medulla, whereas cells binding TPA occurred as single cells or small clumps of cells and resembled Hassall's corpuscles. The cells binding either lectin were identified as epithelial on the basis of ultrastructural features (tonofilaments, desmosomes, and keratohyalin bodies) and resembled Ia+ medullary epithelial cells described previously. An age-related decline in UEA binding was observed, whereas labeling with TPA remained unchanged. On the basis of the labeling patterns obtained with UEA and TPA and the reported specificities of these two lectins, it is suggested that the majority of the fucose detected is associated with type 1 carbohydrate chains.