Transcription-coupled and epigenome-encoded mechanisms direct H3K4 methylation.
Transcription-coupled and epigenome-encoded mechanisms direct H3K4 methylation.
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转录偶联和表观基因组编码机制指导 H3K4 甲基化。
DOI：
10.1101/2021.06.03.446702
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Soichi Inagaki
中科院分区：
文献类型：
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作者：
Satoyo Oya;Mayumi Takahashi;Kazuya Takashima;Tetsuji Kakutani;Soichi Inagaki
组蛋白H3赖氨酸4(H3K4me1/2/3)的单甲基化、双甲基化和三甲基化与转录有关，但H3K4me1/2/3是促进转录还是源于转录仍存在争议。我们之前对拟南芥H3K4去甲基酶的描述表明，H3K4me1在转录中发挥作用。然而，H3K4me1的调控在拟南芥中仍然没有被探索，其中还没有发现H3K4me1的甲基转移酶。在这里，我们鉴定了三种指导H3K4me1的拟南芥甲基转移酶。使用芯片序列和机器学习对其全基因组定位的分析表明，其中一种酶与转录机制合作，而另外两种酶与特定的组蛋白修饰和DNA序列有关。重要的是，在拟南芥和小鼠中也发现了其他H3K4甲基转移酶的这两种定位模式。这些结果表明，H3K4me1/2/3是通过与转录以及其他染色质功能的输入相互作用而建立和维持的，这可能使精细的基因控制成为可能。
Mono-, di-, and trimethylation of histone H3 lysine 4 (H3K4me1/2/3) are associated with transcription, yet it remains controversial whether H3K4me1/2/3 promote or result from transcription. Our previous characterizations ofArabidopsisH3K4 demethylases suggest roles for H3K4me1 in transcription. However, the control of H3K4me1 remains unexplored inArabidopsis, in which no methyltransferase for H3K4me1 has been identified. Here, we identify threeArabidopsismethyltransferases that direct H3K4me1. Analyses of their genome-wide localization using ChIP-seq and machine learning reveal that one of the enzymes cooperates with the transcription machinery, while the other two are associated with specific histone modifications and DNA sequences. Importantly, these two types of localization patterns are also found for the other H3K4 methyltransferases inArabidopsisand mice. These results suggest that H3K4me1/2/3 are established and maintained via interplay with transcription as well as inputs from other chromatin features, presumably enabling elaborate gene control.