Studies on the Physical Characteristics of the Radio-Frequency Atmospheric-Pressure Glow Discharge Plasmas for the Genome Mutation of Methylosinus trichosporium
Studies on the Physical Characteristics of the Radio-Frequency Atmospheric-Pressure Glow Discharge Plasmas for the Genome Mutation of Methylosinus trichosporium
复制标题
射频大气压辉光放电等离子体对毛孢甲基窦菌基因组突变的物理特性研究
DOI：
10.1109/tps.2012.2213274
复制
发表时间：
2012-11-01
影响因子：
1.5
通讯作者：
Xing, Xin-Hui
中科院分区：
文献类型：
--
作者：
Li, He-Ping;Wang, Zhi-Bin;Xing, Xin-Hui
本文首先对射频大气压辉光放电的物理特性进行了模拟和实验研究。实验和模拟结果表明，存在丰富的化学活性物种，例如，电子、氦可吸收物和离子，在放电区域中具有高的数密度。相应的等离子体射流温度在27°C - 39°C的范围内，功率输入低于120 W，这适合于处理生物分子或细胞。然后，以射频等离子体发生器为核心，设计了一台常压室温等离子体诱变系统样机。以发孢甲基弯菌OB 3b为例进行了诱变育种。实验结果表明，RF APGD等离子体源是一种环境友好的微生物基因组突变的高效工具，可用于构建多样性较大的突变体库。
In this paper, both simulations and experiments are conducted to investigate the physical characteristics of the radio-frequency atmospheric-pressure glow discharges (RF APGDs) first. The experimental and modeling results show that there are abundant chemically reactive species, e.g., electrons, helium metastables and ions, with high number densities in the discharge region. The corresponding plasma jet temperatures are in the range of 27°C - 39°C with the power input lower than 120 W, which is suitable for the treatment of the biomolecules or cells. Then, a prototype, which is the so-called atmospheric and room-temperature plasma mutation system, is designed with the RF APGD plasma generator as the core component. As an example, it is used for the mutation breeding of Methylosinus trichosporium OB3b. The experimental results show that the RF APGD plasma source is an environmentally friendly and efficient tool for the genome mutation of microorganisms to construct mutant library with large diversity.