Survey of aflatoxin in dairy cow feed and raw milk in China
Survey of aflatoxin in dairy cow feed and raw milk in China
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DOI：
10.1016/j.foodcont.2013.04.008
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发表时间：
2013-11-01
期刊：
影响因子：
6
通讯作者：
Li, S. L.
中科院分区：
文献类型：
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作者：
Han, R. W.;Zheng, N.;Li, S. L.
2010年8月，从中国10个主要产奶省份采集了200份奶牛饲料样品和200份牛奶样品。使用HPLC方法分析饲料样品中的黄曲霉毒素（AF）B1、B2、G1和G2。采用ELISA法检测牛奶样品中的AFM 1。在饲料样品中检出AFB 1和AFB 2，未检出AFG 1和AFG 2。饲料中AFBI含量在0.05-3.53 μ g/kg的占42%，AFB 2含量在0.03 ~ 0.84 μ g/kg的占36%。饲料中AFBI含量显著高于AFB 2含量（P < 0.05），但仍低于欧盟和我国法律的限量（分别为5 μ g/kg和10 μ g/kg）。AF的总含量低于美国法律的限值20 μ g/kg。对于牛奶样品，32.5%的样品检测到AFM 1阳性，范围为5.2-59.6 ng/L，远低于中国和美国的法律的限值500 ng/L。然而，三个样本中的AFM 1含量超过了欧盟法律的限值50 ng/L。我国南北方奶牛饲料样品和牛奶样品中AF含量差异不显著（P> 0.05）。(C)2013爱思唯尔有限公司保留所有权利。
In August 2010, 200 feed samples for dairy cow and 200 milk samples were collected from ten major milk-producing provinces in China. The feed samples were analysed for Aflatoxin (AF) B1, B2, G1 and G2, using the HPLC method. AFM1 in the milk samples was determined using the ELISA method. AFBI and AFB2, but not AFG1 and AFG2 were detected in the feed samples. In the feeds, 42% of the samples contained AFBI in the range of 0.05-3.53 mu g/kg, and 36% of the samples were detected positive for AFB2, with the content ranging from 0.03 mu g/kg to 0.84 mu g/kg. The content of AFBI was significantly (P < 0.05) higher than that of AFB2 in the feeds, but it was still below the legal limits of 5 pz/kg (in EU) and 10 mu g/kg (in China), respectively. The total content of AFs was below the U.S. legal limit of 20 mu g/kg. For the milk samples, 32.5% were detected positive for AFM1, in the range of 5.2-59.6 ng/L, far below the legal limit of 500 ng/L in China and the US. However, three samples contained AFM1 at the levels of exceeding 50 ng/L of the EU legal limit. Furthermore, there was no significant (P> 0.05) difference between the north and the south of China in the AF contents in both the dairy cow feed samples and the milk samples. (C) 2013 Elsevier Ltd. All rights reserved.