Evidence for dynorphin-A as a neurotransmitter in rat hippocampus.
Evidence for dynorphin-A as a neurotransmitter in rat hippocampus.
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强啡肽 A 作为大鼠海马神经递质的证据。
DOI：
10.1016/0024-3205(83)90432-0
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发表时间：
1983
期刊：
影响因子：
6.1
通讯作者：
Bloom,FE
中科院分区：
文献类型：
--
作者：
Chavkin,C;Bakhit,C;Bloom,FE
采用反相高效液相色谱法（C18-HPLC）分离海马强啡肽A免疫反应性（DynA-IR）的分子形式。与合成的Dyn A（1 - 17）共洗脱的肽占总Dyn A-IR的20%。通过C18-HPLC分离释放的肽，并显示含有与合成Dyn A（1 - 17）共洗脱的Dyn A-IR。这些观察结果与Dyn A（1 - 17）在海马中作为突触处的神经递质的假设一致。
The molecular forms of Dynorphin A immunoreactivity (Dyn A-IR) detected in hippocampus were resolved by reverse-phase high pressure liquid chromatography (C18-HPLC). Peptide co-eluting with synthetic Dyn A (1–17) represents 20% of the total Dyn A-IR. Dyn A-IR was released from hippocampal slices in vitro by a Ca++-dependent mechanism stimulated by superfusion with K+, kainate or veratrine. Released peptides were resolved by C18-HPLC and shown to contain Dyn A-IR co-eluting with synthetic Dyn A (1–17). These observations are consistent with the hypothesis that Dyn A (1–17) acts as a neurotransmitter at synapses in hippocampus.