Evolutionary analysis of three gibberellin oxidase genesin rice, Arabidopsis, and soybean
Evolutionary analysis of three gibberellin oxidase genesin rice, Arabidopsis, and soybean
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DOI：
10.1016/j.gene.2010.10.010
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发表时间：
2011-02-15
期刊：
影响因子：
3.5
通讯作者：
Zhu, Baoge
中科院分区：
文献类型：
--
作者：
Han, Fengming;Zhu, Baoge
GAs是一种植物激素，在植物的生长发育中起着重要的作用。GA2ox、GA3ox和GA20ox是GA生物合成过程中的三个关键酶。这些酶属于20G-Fe(II)加氧酶超家族，由不同的基因家族独立编码。到目前为止，植物中GA氧化酶的全基因组比较分析还没有得到彻底的开展。本研究从水稻、拟南芥和大豆中克隆了61个GA氧化酶家族基因，并对这些基因进行了系统发育树的构建、基因结构、基因家族的扩展和功能基序的分析。根据系统发育，大多数GA氧化酶被分为四个亚类，它们反映了功能分类。水稻GA氧化酶基因的内含子/内含子平均长度分析表明，水稻GA氧化酶基因经历了显著的进化差异。节段性重复事件主要存在于大豆基因组中。然而，在水稻和拟南芥中，没有单一的扩展模式表现出显性，这表明这些物种的GA氧化酶基因可能经历了更复杂的进化机制。此外，在GA20ox、GA3ox和GA2ox中发现了特殊的功能基序，这表明不同的功能基序与蛋白质功能的差异有关。综上所述，我们的结果表明GA氧化酶家族基因经历了不同的进化路线，尤其是在单子叶-双子叶分裂时期，拟南芥和大豆都发生了动态进化。皇冠版权所有(C)2010由爱思唯尔出版。保留所有权利。
GAs are plant hormones that play fundamental roles in plant growth and development. GA2ox, GA3ox, and GA20ox are three key enzymes in GA biosynthesis. These enzymes belong to the 20G-Fe (II) oxygenase superfamily and are independently encoded by different gene families. To date, genome-wide comparative analyses of GA oxidases in plant species have not been thoroughly carried out. In the present work, 61 GA oxidase family genes from rice (Oryza sativa), Arabidopsis, and soybean (Glycine max) were identified and a full study of these genes including phylogenetic tree construction, gene structure, gene family expansion and analysis of functional motifs was performed. Based on phylogeny, most of the GA oxidases were divided into four subgroups that reflected functional classifications. Intron/intron average length of GA oxidase genes in rice analysis revealed that GA oxidase genes in rice experienced substantial evolutionary divergence. Segmental duplication events were mainly found in soybean genome. However, in rice and Arabidopsis, no single expansion pattern exhibited dominance, indicating that GA oxidase genes from these species might have been subjected to a more complex evolutionary mechanism. In addition, special functional motifs were discovered in GA20ox, GA3ox, and GA2ox, which suggested that different functional motifs are associated with differences in protein function. Taken together our results suggest that GA oxidase family genes have undergone divergent evolutionary routes, especially at the monocot-dicot split with dynamic evolution occurring in Arabidopsis thaliana and soybean. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.