The dynamic impact of CpG methylation in DNA
The dynamic impact of CpG methylation in DNA
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DOI：
10.1021/bi9917636
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发表时间：
2000-04-25
期刊：
影响因子：
2.9
通讯作者：
Drobny, GP
中科院分区：
文献类型：
--
作者：
Geahigan, KB;Meints, GA;Drobny, GP
Solid-state deuterium NMR is used to investigate perturbations of the local, internal dynamics in the EcoRI restriction binding site, -GAATTC- induced by cytidine methylation. Methylation of the cytidine base in this sequence is known to suppress hydrolysis by the EcoRI restriction enzyme. Previous solid-state deuterium NMR studies have detected large amplitude motions of the phosphate-sugar backbone at the AT-CG junction of the unmethylated DNA sequence. This study shows that methylation of the cytidine base in a CpG dinucleotide reduces the amplitudes of motions of the phosphate-sugar backbone. These observations suggest a direct link between suppression of the amplitudes of localized, internal motions of the sugar-phosphate backbone of the DNA and inhibition of restriction enzyme cleavage.
