Effect of paraquat on plasma fibronectin, serum free hydroxyproline, serum ceruloplasmin and lung collagen content in monkeys.
Effect of paraquat on plasma fibronectin, serum free hydroxyproline, serum ceruloplasmin and lung collagen content in monkeys.
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百草枯对猴血浆纤连蛋白、血清游离羟脯氨酸、血清铜蓝蛋白和肺胶原含量的影响。
DOI：
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发表时间：
1987
影响因子：
2
通讯作者：
K. Sakaguchi
中科院分区：
文献类型：
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作者：
T. Masaoka;F. Akahori;S. Arai;K. Sakaguchi
本研究的目的是表征百草枯对猴子的毒性，并确定使用猴子作为动物模型来研究百草枯引起的人类肺纤维化的可行性。将16只3.5岁以上、体重3.2kg～10.2kg的日本猴(Macaca fuscata)随机分为两组。他们以 2.0 mg/kg 体重（12 只猴子）的剂量注射（皮下）或皮下注射百草枯二氯化物（PQ）。生理盐水，剂量为0.2ml/kg体重（对照组，4只猴子），每两天一次，连续四到五次。 PQ 治疗组中的 8 只猴子 (66.7%) 在第 11 至 35 天因亚慢性 PQ 毒性而死亡。PQ 治疗组中的 4 只猴子 (33.3%) 和对照组的所有 4 只猴子在 66 天的观察期中存活下来。第66天，处死所有存活的猴子，检查可能的组织病理学变化，并测定肺羟脯氨酸含量。我们的结果表明游离羟脯氨酸和血浆纤连蛋白的浓度没有显着变化。与对照组相比，PQ 治疗组猴子的血清铜蓝蛋白从第 14 天到第 21 天显着增加。此外，与对照组相比，PQ 治疗组中过期猴子和存活猴子的总肺胶原蛋白显着升高。猴子可以为研究人类百草枯中毒的机制和治疗提供广泛的机会。
The purpose of this study was to characterize paraquat toxicity in monkeys and to determine the feasibility of using the monkey as an animal model for the study of paraquat-induced pulmonary fibrosis in humans. Sixteen Japanese monkeys (Macaca fuscata), more than 3.5 years of age, with bodyweight ranging from 3.2 kg to 10.2 kg, were randomly divided into two groups. They were administered paraquat dichloride (PQ) by injection (s.c.) at a dose of 2.0 mg/kg bodyweight (12 monkeys) or s.c. saline, at a dose of 0.2 ml/kg bodyweight (control group, 4 monkeys), every two days for a period of four to five times. Eight monkeys (66.7%) from the PQ treatment groups expired due to subchronic PQ toxicity from days 11 to 35. Four monkeys (33.3%) from the PQ treatment group and all four monkeys from the control group survived the observation period of 66 days. On day 66, all of the surviving monkeys were sacrificed and examined for possible histopathological changes and the lung hydroxyproline content was determined. Our results indicate that the concentration of free hydroxyproline and plasma fibronectin did not vary significantly. The serum ceruloplasmin for the monkeys of the PQ treatment groups was significantly increased from day 14 to 21, compared to the control group. Also the total lung collagen of both the expired and surviving monkeys in the PQ treatment groups was elevated significantly, compared to the control group. The monkeys can provide extensive opportunities for research on the mechanism and the treatment of PQ poisoning in man.
影响因子：
2.9
作者：
JAMALL, IS;FINELLI, VN;HEE, SSQ
通讯作者：
HEE, SSQ