Morphogenesis of the anterior segment in the zebrafish eye.
Morphogenesis of the anterior segment in the zebrafish eye.
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DOI：
10.1186/1471-213x-5-12
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发表时间：
2005-06-28
影响因子：
--
通讯作者：
Link BA
中科院分区：
文献类型：
--
作者：
Soules KA;Link BA
眼前节对于将入射光聚焦到神经视网膜上和调节眼内压至关重要。它由角膜、透镜、虹膜、睫状体和虹膜角膜角的高度特化组织组成。在发育过程中，来自不同胚胎谱系的细胞相互作用形成眼前节。这些细胞的异常迁移、增殖、分化或存活导致前段疾病，例如角膜营养不良、透镜白内障和青光眼。斑马鱼是研究发育和疾病的遗传学和细胞生物学的强大模式生物。为了给眼前节发育的遗传学研究奠定基础，我们描述了斑马鱼眼前节结构的形态发生。与其他脊椎动物一样，斑马鱼的眼前节来源于不同的来源，包括表面外胚层、眼周间充质和神经上皮。类似地，前节组织分化的相对时间也与其他脊椎动物保持一致。然而，斑马鱼眼前节的几个形态发生特征与高等脊椎动物不同。这些包括透镜分层而不是内陷，虹膜肌肉和睫状褶皱缺乏，以及虹膜角膜角的组织改变。此外，在斑马鱼的前节内存在着大量的背腹差异。累积起来，我们的解剖学研究结果提供了一个参考点，利用斑马鱼的遗传研究的机制，发展和维护的前段。
The ocular anterior segment is critical for focusing incoming light onto the neural retina and for regulating intraocular pressure. It is comprised of the cornea, lens, iris, ciliary body, and highly specialized tissue at the iridocorneal angle. During development, cells from diverse embryonic lineages interact to form the anterior segment. Abnormal migration, proliferation, differentiation, or survival of these cells contribute to diseases of the anterior segment such as corneal dystrophy, lens cataract, and glaucoma. Zebrafish represent a powerful model organism for investigating the genetics and cell biology of development and disease. To lay the foundation for genetic studies of anterior segment development, we have described the morphogenesis of this structure in zebrafish. As in other vertebrates, the zebrafish anterior segment derives from diverse origins including surface ectoderm, periocular mesenchyme, and neuroepithelium. Similarly, the relative timing of tissue differentiation in the anterior segment is also conserved with other vertebrates. However, several morphogenic features of the zebrafish anterior segment differ with those of higher vertebrates. These include lens delamination as opposed to invagination, lack of iris muscles and ciliary folds, and altered organization in the iridocorneal angle. In addition, substantial dorsal-ventral differences exist within the zebrafish anterior segment. Cumulatively, our anatomical findings provide a reference point to utilize zebrafish for genetic studies into the mechanisms of development and maintenance of the anterior segment.