Ultrastructural characteristics of Sin Nombre virus, causative agent of hantavirus pulmonary syndrome
Ultrastructural characteristics of Sin Nombre virus, causative agent of hantavirus pulmonary syndrome
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DOI：
10.1007/bf01323234
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发表时间：
1995-01-01
影响因子：
2.7
通讯作者：
Zaki, SR
中科院分区：
文献类型：
--
作者：
Goldsmith, CS;Elliott, LH;Zaki, SR
1993年5月至6月，在美国西南部爆发了严重的、往往是致命的肺部疾病之后，出现了一种以前未被认识到的疾病——汉坦病毒肺综合征。我们现在通过感染Vero E6细胞的薄片电镜和免疫电镜研究了病原体sinnombre病毒（SNV）的形态特征。SNV病毒粒子大致为球形，平均直径为112 nm。它们有一个相当致密的包膜和紧密相对的精细表面突起，长度为7纳米。丝状核衣壳存在于病毒粒子内。病毒包涵体存在于感染细胞的细胞质中；这些颗粒状或丝状，取决于断面的平面。所有这些特征都与已发表的其他汉坦病毒的描述相似；然而，与所有其他汉坦病毒和几乎所有布尼亚病毒科的其他成员病毒在光滑的胞质膜上出芽不同，SNV几乎完全发生在感染细胞的质膜上。病毒出芽与长直径28 nm的管状突起的形成有关。胞质膜上偶见直径为47纳米的细长病毒样颗粒萌发。免疫电镜显示，病毒抗原定位在病毒粒子、包涵体和与病毒粒子形态发生相关的管状突起上。
A previously unrecognized disease, hantavirus pulmonary syndrome, was described following an outbreak of severe, often lethal, pulmonary illness in the southwestern United States in May-June, 1993. We have now studied the morphologic features of the causative agent, Sin Nombre virus (SNV), by thin section electron microscopy and immunoelectron microscopy of infected Vero E6 cells. SNV virions were roughly spherical and had a mean diameter of 112 nm. They had a rather dense envelope and closely apposed fine surface projections, 7 nm in length. Filamentous nucleocapsids were present within virions. Viral inclusion bodies were present in the cytoplasm of infected cells; these appeared granular or filamentous, depending on the plane of section. All of these characteristics were similar to published descriptions of other hantaviruses; however, unlike all other hantaviruses and virtually all other member viruses of the family Bunyaviridae which bud upon smooth intracytoplasmic membranes, SNV budding occurred almost entirely upon the plasma membrane of infected cells. Virus budding was associated with the formation of long 28 nm diameter tubular projections. Occasional elongated 47 nm diameter virus-like particles were seen to bud upon intracytoplasmic membranes. As shown by immunoelectron microscopy, viral antigens were localized over virions, inclusions, and tubular projections associated with virion morphogenesis.