Co-contaminant effects on 1,4-dioxane biodegradation in packed soil column flow-through systems.
Co-contaminant effects on 1,4-dioxane biodegradation in packed soil column flow-through systems.
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DOI：
10.1016/j.envpol.2018.09.018
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发表时间：
2018-12
影响因子：
8.9
通讯作者：
Linduo Zhao;Xia Lu;Alexandra L. Polasko;Nicholas W. Johnson;Yu Miao;Ziming Yang;Shaily Mahendra;
中科院分区：
文献类型：
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作者：
Linduo Zhao;Xia Lu;Alexandra L. Polasko;Nicholas W. Johnson;Yu Miao;Ziming Yang;Shaily Mahendra;
研究了1，4-二氧六环在填充石英柱和土壤柱中的生物降解。共污染物，特别是三氯乙烯（TCE），1，1-二氯乙烯（1，1-DCE），和铜（Cu 2+）离子的抑制作用进行了研究，无论是与1，4-二氧六环降解菌Pseudonocardia dioxanivoransCB 1190的生物强化。结果表明，CB 1190细胞很容易生长和定殖的列，导致显着降解的1，4-二氧六环在好氧条件下。1，4-二氧六环的降解也在原生土壤（无生物强化）中观察到，该土壤先前已对共污染物三氯乙烯和1，1-二氯乙烷进行了强化还原脱氯处理。用CB 1190对土壤进行生物强化，在进水浓度为3-10 mg L− 1,1，4-二氧六环和停留反应时间为40-80 h时几乎完全降解，但共污染物1，1-DCE和Cu 2+离子（高达10 mg L−1）的存在部分抑制了未处理和生物强化土壤柱中的1，4-二氧六环降解。然而，在柱流通系统中的抑制作用比先前在无菌培养物中观察到的抑制作用严重得多，在相同的共污染物浓度下，无菌培养物显示出接近完全的抑制。这些观察结果表明，土壤微生物对1，1-DCE和Cu 2+的毒性在填充土壤流通系统中的敏感性较低，因此对预测生物降解潜力和开发可持续的，具有成本效益的技术原位修复1，4-二氧六环污染的土壤和地下水具有重要意义。
Biodegradation of 1,4-dioxane was examined in packed quartz and soil column flow-through systems. The inhibitory effects of co-contaminants, specifically trichloroethene (TCE), 1,1-dichloroethene (1,1-DCE), and copper (Cu2+) ions, were investigated in the columns either with or without bioaugmentation with a 1,4-dioxane degrading bacteriumPseudonocardia dioxanivoransCB1190. Results indicate that CB1190 cells readily grew and colonized in the columns, leading to significant degradation of 1,4-dioxane under oxic conditions. Degradation of 1,4-dioxane was also observed in the native soil (without bioaugmentation), which had been previously subjected to enhanced reductive dechlorination treatment for co-contaminants TCE and 1,1-DCE. Bioaugmentation of the soil with CB1190 resulted in nearly complete degradation at influent concentrations of 3–10 mg L−11,4-dioxane and a residence reaction time of 40–80 h, but the presence of co-contaminants, 1,1-DCE and Cu2+ions (up to 10 mg L−1), partially inhibited 1,4-dioxane degradation in the untreated and bioaugmented soil columns. However, the inhibitory effects were much less severe in the column flow-through systems than those previously observed in planktonic cultures, which showed near complete inhibition at the same co-contaminant concentrations. These observations demonstrate a low susceptibility of soil microbes to the toxicity of 1,1-DCE and Cu2+in packed soil flow-through systems, and thus have important implications for predicting biodegradation potential and developing sustainable, cost-effective technologies forin situremediation of 1,4-dioxane contaminated soils and groundwater.