Transcriptomic Analysis of Leaf Sheath Maturation in Maize
Transcriptomic Analysis of Leaf Sheath Maturation in Maize
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DOI：
10.3390/ijms20102472
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发表时间：
2019-05-02
影响因子：
5.6
通讯作者：
Li, Pinghua
中科院分区：
文献类型：
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作者：
Dong, Lei;Qin, Lei;Li, Pinghua
叶的形态发育对植物的结构和产量有很大的影响。玉米叶由叶片、叶舌和叶鞘组成。虽然已经对发育中的玉米叶片的纵向轴沿着组织进行了广泛的转录谱分析，但是对于在支持叶片中起重要作用的叶鞘组织的转录动态知之甚少。使用全面的转录组数据集，我们证明了叶鞘转录组在成熟过程中动态变化，在鞘成熟的最早阶段构建基本细胞结构，并过渡到细胞壁生物合成和修饰。在叶鞘组织成熟的最后阶段，转录组再次随着光合作用和木质素生物合成而改变。玉米叶片的不同组织在其生物学功能方面是高度特化的，并且我们鉴定了15个基因，与它们在叶片中的表达相比，它们在叶鞘中以显著更高的水平表达，包括BOP 2同源物GRMZM 2G026556和GRMZM 2G022606、DOGT 1（GRMZM2G403740）和来自B3结构域、C2H2锌指和同源盒基因家族的转录因子，暗示这些基因在鞘成熟和器官特化中。
The morphological development of the leaf greatly influences plant architecture and crop yields. The maize leaf is composed of a leaf blade, ligule and sheath. Although extensive transcriptional profiling of the tissues along the longitudinal axis of the developing maize leaf blade has been conducted, little is known about the transcriptional dynamics in sheath tissues, which play important roles in supporting the leaf blade. Using a comprehensive transcriptome dataset, we demonstrated that the leaf sheath transcriptome dynamically changes during maturation, with the construction of basic cellular structures at the earliest stages of sheath maturation with a transition to cell wall biosynthesis and modifications. The transcriptome again changes with photosynthesis and lignin biosynthesis at the last stage of sheath tissue maturation. The different tissues of the maize leaf are highly specialized in their biological functions and we identified 15 genes expressed at significantly higher levels in the leaf sheath compared with their expression in the leaf blade, including the BOP2 homologs GRMZM2G026556 and GRMZM2G022606, DOGT1 (GRMZM2G403740) and transcription factors from the B3 domain, C2H2 zinc finger and homeobox gene families, implicating these genes in sheath maturation and organ specialization.