DL-2-amino-4-phosphonobutyric acid does not eliminate "ON" responses in the visual system of goldfish.
DL-2-amino-4-phosphonobutyric acid does not eliminate "ON" responses in the visual system of goldfish.
复制标题
DL-2-氨基-4-磷酸丁酸不会消除金鱼视觉系统中的“ON”反应。
DOI：
10.1073/pnas.88.9.3787
复制
发表时间：
1991
影响因子：
11.1
通讯作者：
Powers,MK
中科院分区：
文献类型：
--
作者：
DeMarcoJr,PJ;Bilotta,J;Powers,MK
DL-2-氨基-4-磷酸丁酸 (APB) 抑制视网膜 ON 通路的活性。通常认为 ON 通路的丢失将导致视觉系统中 ON 反应的丢失。我们通过记录眼内注射 APB 前后完整金鱼 (Carassius auratus) 视神经的活动来测试这一假设。将全神经对光增量和减量的反应与视网膜电图反应和顶盖诱发电位进行比较。 APB 严重降低了视网膜电图 b 波的幅度，但使视神经和顶盖的 ON 和 OFF 响应保持完整，尽管敏感性有所降低。我们得出的结论是，APB 并不能完全消除视觉系统中的 ON 反应，至少在金鱼中是这样。 APB 的选择性和有效性必须在其他物种中进行评估，然后才能依赖该试剂作为理解 ON 和 OFF 通路在视觉功能中的作用的有用工具。
DL-2-Amino-4-phosphonobutyric acid (APB) suppresses activity in retinal ON pathways. It is generally assumed that loss of the ON pathway would result in loss of ON responses in the visual system. We tested this assumption by recording activity from the optic nerves of intact goldfish (Carassius auratus) before and after intraocular injection of APB. Whole-nerve responses to increments and decrements of light were compared to electroretinogram responses and to tectal evoked potentials. APB severely reduced the amplitude of the electroretinogram b-wave but left ON and OFF responses from the optic nerve and tectum intact, although decreased in sensitivity. We conclude that APB does not completely eliminate ON responses in the visual system, at least in goldfish. The selectivity and effectiveness of APB must be evaluated in other species before this agent can be relied upon as a useful tool in understanding the roles of ON and OFF pathways in visual function.