Impact of a new refrigerator for the preservation of whole blood.
Impact of a new refrigerator for the preservation of whole blood.
复制标题
新冰箱对保存全血的影响。
DOI：
10.1016/j.jss.2010.09.011
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
J. Hanaoka
中科院分区：
文献类型：
--
作者：
M. Kanamoto;M. Shimada;T. Utsunomiya;S. Imura;Y. Morine;T. Ikemoto;H. Mori;J. Hanaoka
背景技术近年来，人们开发出一种新型制冷系统，通过频繁地感测物料内部和表面的温度，将物料内部冷却到所需的温度。本研究的目的是评估这一新系统在全血保存中的有用性。材料和方法研究1（人血）：用CPDA 对来自健康志愿者的全血样本进行抗凝。样品被分为两组。 A组全血在普通冰箱中保存，B组全血在新冰箱中保存（4℃，15周）。保存后，氨、乳酸、pH。和 ATP 水平进行了分析。研究2（大鼠实验）：雄性Wistar大鼠的全血样本，保存在普通冰箱（C组）或新型冰箱（D组）中。保存8周后输注全血，比较存活率。 结果研究1：保存后7、9周，B组氨浓度显着低于A组（P < 0.05）。保存后9周，B组乳酸水平显着低于A组（P < 005）。保存后5～13周，B组pH值高于A组（P < 0.05）。保存后5～13周B组ATP水平高于A组。研究2：在急性失血性休克模型大鼠输血研究中，C组存活率显着高于D组（P < 0.05）。结论我们的新型冰箱可用于保存全血，在保存过程中状态良好。
BACKGROUNDA new refrigerating system has been recently developed, which can cool the inside of a material to the required temperature by frequently sensing the temperature of both the inside and the surface of the materials. The purpose of this study was to evaluate the usefulness of this new system in whole blood preservation.MATERIALS AND METHODSStudy 1 (human blood): Whole blood samples from healthy volunteers were anticoagulated with CPDA. The samples were divided into two groups. Group A, whole blood preserved in ordinary refrigerator and Group B, whole blood preserved in new refrigerator (4°C, 15 wk). After preservation, ammonia, lactate, pH. and ATP levels were analyzed. Study 2 (rat experiment): Whole blood samples from male Wistar rats, stored in ordinary refrigerator (Group C) or new refrigerator (Group D). Whole blood was transfused after 8-wk storage and survival was compared.RESULTSStudy 1: The ammonia concentrations in Group B were significantly lower than those in Group A at 7 and 9 wk after preservation (P < 0.05). The lactate levels in Group B were significantly lower than those in Group A at 9 wk after preservation (P < 005). The pH levels in Group B were higher than those in Group A at 5 to 13 wk after preservation (P < 0.05). The ATP levels in Group B were higher than those in Group A at 5 to 13 wk after preservation. Study 2: In rat studies using blood transfusion in acute hemorrhagic shock model, survival rate in Group C was significantly higher than those in Group D (P < 0.05).CONCLUSIONSOur new refrigerator is useful in preserving whole blood with good condition during preservation.
红细胞囊泡形成——一种常见的膜生理事件。
DOI：
--
发表时间：
1986
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Wagner,GM;Chiu,DT;Yee,MC;Lubin,BH
通讯作者：
Lubin,BH