Expression of the MAGE-A4 and NY-ESO-1 cancer-testis antigens and T cell infiltration in non-small cell lung carcinoma and their prognostic significance.
Expression of the MAGE-A4 and NY-ESO-1 cancer-testis antigens and T cell infiltration in non-small cell lung carcinoma and their prognostic significance.
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DOI：
10.3892/ijo.28.5.1089
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发表时间：
2006-05
影响因子：
5.2
通讯作者：
N. Yoshida;H. Abe;T. Ohkuri;D. Wakita;Masayoshi Sato;D. Noguchi;M. Miyamoto;T. Morikawa;S. Kondo;H. Ikeda;T. Nishimura
中科院分区：
文献类型：
--
作者：
N. Yoshida;H. Abe;T. Ohkuri;D. Wakita;Masayoshi Sato;D. Noguchi;M. Miyamoto;T. Morikawa;S. Kondo;H. Ikeda;T. Nishimura
癌睾丸（CT）抗原被认为是一组极具吸引力的癌症免疫治疗靶点，因为它们在各种恶性肿瘤中表达，但在正常成人组织中仅在睾丸中表达。为了评估MAGE-A4和y - eso -1这两个家族成员抗原在非小细胞肺癌（NSCLC）中用于癌症疫苗的潜力，我们检测了这些抗原的表达和肿瘤组织中的T细胞浸润，并评估了它们的预后意义。157例非小细胞肺癌患者进行了研究。逆转录pcr检测MAGE-A4和NY-ESO-1的表达。免疫组化检测NY-ESO-1表达及T细胞浸润情况。同时进行生存分析。141例NSCLC中有40例（28.4%）表达MAGE-A4, 157例中有13例（8.3%）表达new - eso -1。这两种CT抗原在鳞状细胞癌（SCC）中比在腺癌中更频繁地表达。发现MAGE-A4表达与晚期癌症患者生存呈负相关。CD4+和CD8+ T细胞联合浸润肿瘤巢预示着更好的生存率。肿瘤中淋巴细胞浸润与抗原表达无相关性。晚期组MAGE-A4表达及T细胞浸润可提供预后信息。最后，这些CT抗原，特别是MAGE-A4，可能代表非小细胞肺癌患者癌症免疫治疗的潜在靶点。
Cancer-testis (CT) antigens were identified as a group of highly attractive targets for cancer immunotherapy because of their expression in a variety of malignant tumors but solely in the testis among the normal adult tissues. To evaluate the potential of two members of this family, MAGE-A4 and NY-ESO-1 antigens, for cancer vaccine in non-small cell lung carcinoma (NSCLC), we examined the expression of these antigens and T cell infiltration in tumor tissue, and evaluated their prognostic significance. One hundred fifty-seven patients with NSCLC were studied. Reverse transcription-PCR was performed to evaluate MAGE-A4 and NY-ESO-1 expression. Immunohistochemistry was performed for NY-ESO-1 expression and T cell infiltration into the tumor site. Survival analysis was also performed. MAGE-A4 and NY-ESO-1 were expressed in 40 of 141 (28.4%) and 13 of 157 (8.3%) NSCLC respectively. Both CT antigens were more frequently expressed in squamous cell carcinoma (SCC) than in adenocarcinoma. An inverse correlation was found between MAGE-A4 expression and patient survival in advanced stage cancers. Combined infiltration of both CD4+ and CD8+ T cells into tumor nest predicted better survival. There was no correlation, however, between lymphocyte infiltration and antigen expression in the tumor. MAGE-A4 expression in advanced group and T cell infiltration may provide prognostic information. Lastly, these CT antigens, especially MAGE-A4, may represent potential targets for cancer immunotherapy in patients with NSCLC.