Hypoxia and vascular endothelial growth factor expression in human squamous cell carcinomas using pimonidazole as a hypoxia marker.
Hypoxia and vascular endothelial growth factor expression in human squamous cell carcinomas using pimonidazole as a hypoxia marker.
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发表时间：
1998-09
期刊：
影响因子：
11.2
通讯作者：
J. Raleigh;Dennise P. Calkins-Adams;L. Rinker;C. A. Ballenger;M. Weissler;W. Fowler;D. Novotny;M. Varia
中科院分区：
文献类型：
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作者：
J. Raleigh;Dennise P. Calkins-Adams;L. Rinker;C. A. Ballenger;M. Weissler;W. Fowler;D. Novotny;M. Varia
人类肿瘤中的缺氧与预后不良有关，但这种联系背后的分子机制尚不清楚。一种可能性是，缺氧通过诱导血管内皮生长因子(VEGF)诱导以及相关的血管生成和转移而与恶性进展有关。目前的临床研究在逐个细胞的基础上测量人类鳞状细胞癌中的缺氧和血管内皮生长因子的表达，以验证缺氧和血管内皮生长因子蛋白表达在人类肿瘤中耦合的假说。对18例宫颈和头颈部浸润性鳞癌患者的肿瘤切片进行免疫组织化学定量图像分析。用缺氧标志物匹莫硝唑检测肿瘤缺氧，用市售抗体检测血管内皮细胞生长因子蛋白表达。低氧与血管内皮生长因子蛋白表达的免疫组织化学定量比较显示，两者之间无相关性。
Hypoxia in human tumors is associated with poor prognosis, but the molecular mechanisms underlying this association are poorly understood. One possibility is that hypoxia is linked to malignant progression through vascular endothelial growth factor (VEGF) induction and the associated angiogenesis and metastasis. The present clinical study measures hypoxia and VEGF expression on a cell-by-cell basis in human squamous cell carcinomas to test the hypothesis that hypoxia and VEGF protein expression are coupled in human tumors. Eighteen patients with invasive squamous cell carcinoma of the uterine cervix and head and neck have been investigated by a quantitative image analysis of immunostained sections from their tumors. The hypoxia marker pimonidazole was used to measure tumor hypoxia, and a commercially available antibody was used to measure VEGF protein expression. A quantitative immunohistochemical comparison of hypoxia and VEGF protein expression revealed no correlation between the two factors.